GRP75/HSPA9B/Mortalin Antibody

Western Blot: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with GRP75/HSPA9B/Mortalin antibody (NBP1-31596) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - GRP75/HSPA9B/Mortalin antibody detects GRP75/HSPA9B/Mortalin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.GRP75/HSPA9B/Mortalin stained by GRP75/HSPA9B/Mortalin antibody (NBP1-31596) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - GRP75/HSPA9B/Mortalin antibody detects GRP75/HSPA9B/Mortalin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse stomach.GRP75/HSPA9B/Mortalin stained by GRP75/HSPA9B/Mortalin antibody (NBP1-31596) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRP75/HSPA9B/Mortalin antibody (NBP1-31596) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Whole cell extract (6 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GRP75 antibody (NBP1-31596) diluted at 1:500.

GRP75/HSPA9B/Mortalin antibody detects GRP75/HSPA9B/Mortalin protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GRP75/HSPA9B/Mortalin stained by GRP75/HSPA9B/Mortalin antibody (NBP1-31596) diluted at 1:500.Blue: Hoechst 33342 staining.

GRP75/HSPA9B/Mortalin antibody detects GRP75/HSPA9B/Mortalin protein at mitochondria in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. GRP75/HSPA9B/Mortalin antibody (NBP1-31596) diluted at 1:500. ^[^GTX]br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GRP75/HSPA9B/Mortalin antibody detects GRP75/HSPA9B/Mortalin protein at mitochondria in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. GRP75/HSPA9B/Mortalin antibody (NBP1-31596) diluted at 1:500. ^[^GTX]br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GRP75/HSPA9B/Mortalin antibody detects GRP75/HSPA9B/Mortalin protein at mitochondria in rat duodenum by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. GRP75/HSPA9B/Mortalin antibody (NBP1-31596) diluted at 1:500. ^[^GTX]br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, Rt, Po, Xp
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

GRP75/HSPA9B/Mortalin Antibody Summary

• Description: Novus Biologicals Rabbit GRP75/HSPA9B/Mortalin Antibody (NBP1-31596) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human GRP75/HSPA9B/Mortalin. The exact sequence is proprietary.
• Localization: Mitochondrion
• Marker: Immortalization Marker
• Predicted Species: Xenopus (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HSPA9
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 74 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (87%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for GRP75/HSPA9B/Mortalin Antibody

• 75 kDa glucose-regulated protein
• CSA
• GRP75
• GRP-75
• GRP75mthsp75
• Heat shock 70 kDa protein 9
• heat shock 70kD protein 9B
• heat shock 70kDa protein 9 (mortalin)
• heat shock 70kDa protein 9B (mortalin-2)
• HSPA9
• HSPA9B
• HSPA9BMGC4500
• Mortalin
• mortalin, perinuclear
• Mortalin-2
• MOT
• MOT2
• mot-2
• MTHSP75
• p66-mortalin
• PBP74
• PBP74CSA
• Peptide-binding protein 74
• stress-70 protein, mitochondrial

Background

Suitable for use in Western Blot. Other applications not tested. Human and mouse Grp 75

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HSPA9