Growth Hormone R Antibody [Unconjugated]

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Recombinant Human Growth Hormone R/GHR Fc Chimera (Catalog #1210‑GR) inhibits Recombinant Human Growth Hormone (Catalog # 1067‑GH) induced proliferation in the Nb2‑11 rat lymphoma cell line in a dose-dependent ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, B/N, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Growth Hormone R Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Growth Hormone R/GHR
Ala27-Tyr264
Accession # P10912
Specificity
Detects human Growth Hormone R/GHR in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant mouse GHR and recombinant rat GHR is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GHR
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • CyTOF-ready
  • Neutralization 0.125-0.5 ug/mL
Publications
Read Publications using
AF1210 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Growth Hormone R Antibody [Unconjugated]

  • GH receptor
  • GHBP
  • GHR
  • growth hormone binding protein
  • Growth Hormone R
  • growth hormone receptor
  • serum binding protein
  • Somatotropin receptor

Background

Growth hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins and somatolactin (1, 2). It is synthesized primarily by somatotropes in the anterior pituitary and is released as an endocrine hormone. Other cells and tissues, including lymphoid tissues, can also produce GH (3). GH is a pleiotropic molecule which can act directly or indirectly via IGF-I, to regulate growth and metabolism as well as enhance T cell survival and thymic functions (1, 2, 4). GH exerts its biological actions by binding to the GH receptor (GHR) that is present in many cell types (1, 2). Human GHR cDNA encodes a 638 amino acid (aa) residue type I transmembrane protein with an 18 aa putative signal peptide, a 246 aa extracellular domain, a 24 aa transmembrane domain and a 350 aa cytoplasmic domain (5). At least two alternatively spliced isoforms of human GHR, lacking the sequence encoded by exon 3, or lacking most of the cytoplasmic domain, also exist (6, 7). Soluble GH-binding proteins corresponding to extracellular domain of the transmembrane proteins can be generated from the membrane proteins (8). Ligation of GHR by GH has been shown to result in receptor dimerization and activation of the JAK/STAT signaling cascade (9). The soluble GHBP has been shown to interfere with GH signaling by competing with the transmembrane receptor of GH. Alternatively, the GHBP has also been shown to enhance GH action by slowing GH clearance (8, 10).

  1. Goffin, V. et al. (1996) Endocrine Rev. 17:385.
  2. Le Roith, D. et al. (2001) Endocrine Rev. 22:53.
  3. Clark, R. (1997) Endocr. Rev. 18:157.
  4. Welniak, L.A. et al. (2002) J. Leukoc. Biol. 71:381.
  5. Leung, D.W. et al. (1987) Nature 330:537.
  6. Stallings-Mann, J.L. et al. (1996) Proc. Nat. Acad. Sci. 93:12394.
  7. Amit, T. et al. (1997) Endocr. Metab. 82:3813.
  8. Ross, R.J.M., et al. (1997) Molecular Endocrinology 11:265.
  9. Carter-Su, C. et al. (1996) Annu. Rev. Physiol. 58:187.
  10. Postel-Vinay, M.C. and J. Finidori (1995) Eur. J. Endocrinol. 133:654.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Growth Hormone R Antibody (AF1210)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Flow Cytometry, Neutralization.


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Flow Cytometry
(1)
Neutralization
(1)
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(2)
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Secondary Antibodies

 

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Biotin BAF1210

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Bioinformatics

Gene Symbol GHR
Uniprot