Growth Hormone R Antibody (3A12)

Proximity Ligation Assay: Growth Hormone R Antibody (3A12) [H00002690-M01] - Analysis of protein-protein interactions between STAT5A and GHR. HeLa cells were stained with anti-STAT5A rabbit purified polyclonal 1:1200 and anti-GHR mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Sandwich ELISA: Growth Hormone R Antibody (3A12) [H00002690-M01] - Detection limit for recombinant GST tagged GHR is approximately 0.1ng/ml as a capture antibody.

Product Discontinued

Growth Hormone R Antibody (3A12) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: GHR (NP_000154, 19 a.a. ~ 118 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. FSGSEATAAILSRAPWSLQSVNPGLKTNSSKEPKFTKCRSPERETFSCHWTDEVHHGTKNLGPIQLFYTRRNTQEWTQEWKECPDYVSAGENSCYFNSSF
• Specificity: GHR - growth hormone receptor
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GHR
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Proximity Ligation Assay
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Growth Hormone R Antibody (3A12)

• GH receptor
• GHBP
• GHR
• growth hormone binding protein
• Growth Hormone R
• growth hormone receptor
• serum binding protein
• Somatotropin receptor

Background

This gene encodes a protein that is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization and the activation of an intra- and intercellular signal transduction pathway leading to growth. A common alternate allele of this gene, called GHRd3, lacks exon three and has been well-characterized. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature. Other splice variants, including one encoding a soluble form of the protein (GHRtr), have been observed but have not been thoroughly characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GHR
• Entrez:
  • 2690(Human)
• OMIM:
  • 262500(Human)
• Uniprot:
  • NP_000154(Human)