Growth Hormone Antibody (222540)

Western blot shows lysates of rat brain tissue. PVDF membrane was probed with 2 µg/mL of Rat Growth Hormone Monoclonal Antibody (Catalog # MAB1566) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Growth Hormone at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

• Reactivity: Rt
• Applications: WB
• Clone: 222540
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Growth Hormone Antibody (222540) Summary

• Immunogen: E. coli-derived recombinant rat Growth Hormone
  Phe27-Phe216
  Accession # P01244
• Specificity: Detects rat Growth Hormone in direct ELISAs and Western blots. Does not cross‑react with recombinant human Growth Hormone.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: GH1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Growth Hormone Antibody (222540)

• GH1
• GHB5
• GHIGHD1B
• GHN
• GH-N
• GHNGrowth hormone
• GH-Nsomatotropin
• growth hormone 1Pituitary growth hormone
• Growth Hormone
• hGH-N
• IGHD1A
• IGHD2
• Somatotropin

Background

Growth Hormone, also known as somatotropin, is synthesized primarily in the anterior pituitary. It is a pleiotropic cytokine that exerts its biological actions by binding to the transmembrane GH receptor which is present in many cell types.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Growth Hormone Antibody (MAB1566)(4)

Publications using MAB1566

• MOROKI T, MATSUO S, HATAKEYAMA H et al. Databases for technical aspects of immunohistochemistry-2021 update Journal of Toxicologic Pathology 2/24/2021 [PMID: 33976473]
• Masaaki Yamamoto, Anat Ben-Shlomo, Hiraku Kameda, Hidenori Fukuoka, Nan Deng, Yan Ding et al. Somatostatin receptor subtype 5 modifies hypothalamic-pituitary-adrenal axis stress function JCI Insight 2018-10-04 [PMID: 30282821]
• Haojie Zhang, Qien Qi, Ting Chen, Junyi Luo, Qianyun Xi, Qingyan Jiang et al. Age-Related Changes in MicroRNA in the Rat Pituitary and Potential Role in GH Regulation International Journal of Molecular Sciences 2018-07-15 [PMID: 30011963]
• Haojie Zhang, Qien Qi, Ting Chen, Junyi Luo, Qianyun Xi, Qingyan Jiang et al. Age-Related Changes in MicroRNA in the Rat Pituitary and Potential Role in GH Regulation International Journal of Molecular Sciences 2018-07-15 [PMID: 30011963] (Western Blot, Rat)

Bioinformatics

• Gene Symbol: GH1
• Uniprot:
  • P01244()