GRB2 Antibody (M331)

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IP
• Clone: M331
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

GRB2 Antibody (M331) Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: Clone (M331) was generated from a full length recombinant rat Grb2 protein. This sequence has high homology to human and mouse Grb2. [UniProt# P62994]
• Specificity: This antibody detects a 24 kDa protein corresponding to the molecular mass of Grb2 on SDS-PAGE immunoblots of rat PC12.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GRB2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunoprecipitation 1:50
  • Western Blot 1:1000
• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Alternate Names for GRB2 Antibody (M331)

• abundant SRC homology
• Adapter protein GRB2
• ASH
• EGFRBP-GRB2
• epidermal growth factor receptor-binding protein GRB2
• GRB2
• Grb3-3
• growth factor receptor-bound protein 2
• growth factor receptor-bound protein 3
• HT027
• MST084
• MSTP084
• NCKAP2
• Protein Ash
• SH2/SH3 adapter GRB2

Background

The growth factor bound protein 2 (GRB2) is a 25 kDa cytosolic protein that contains one src homology (SH2) domain and two SH3 domains (1). GRB2 associates with Tyrosine phosphorylated EGFRs and PDGFRs via its SH2 domain. GRB2 also plays a crucial role in two separate insulin pathways leading from the insulin receptor to the Ras protein thus affecting mitogenic signaling (2). Additionally GBR2, a bifunctional adaptor protein, recruits Sos for Ras activation and subsequent signal transmission (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GRB2