GRASP55 Overexpression Lysate (Native)

Images

 
Western Blot: GRASP55 Overexpression Lysate (Adult Normal) [NBL1-11203] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GRASP55.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

GRASP55 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC202946 and based on accession number NM_015530. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens golgi reassembly stacking protein 2, 55kDa (GORASP2), mRNA.
Gene
GORASP2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for GRASP55 Overexpression Lysate (Native)

  • Golgi phosphoprotein 6
  • golgi reassembly stacking protein 2, 55 kDa
  • golgi reassembly stacking protein 2, 55kDa
  • Golgi reassembly-stacking protein of 55 kDa
  • GOLPH6DKFZp434D156
  • GRASP55FLJ13139
  • GRS2Golgi reassembly-stacking protein 2
  • p59

Background

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is involved in establishing the stacked structure of the Golgi apparatus. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB500-517
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-46218
Species: Hu
Applications: WB, IHC
NBP1-84084
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-02340
Species: Hu, Mu, Rt, Mk
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-80959
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
MAB7500
Species: Hu
Applications: WB, ICC
NBP2-02665
Species: Hu, Rt, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P, ICC
NB110-60490
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
H00002288-M01
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC-P, IP
AF2685
Species: Hu
Applications: WB, Simple Western, IHC
NB120-2928
Species: Hu, Mu, Rt, Pm, Rb, Sh
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP1-49045
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
NBP1-91228
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
MAB3915
Species: Hu, Mu
Applications: WB
NBP2-67817
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBL1-11203
Species: Hu
Applications: WB

Publications for GRASP55 Lysate (NBL1-11203) (0)

There are no publications for GRASP55 Lysate (NBL1-11203).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for GRASP55 Lysate (NBL1-11203) (0)

There are no reviews for GRASP55 Lysate (NBL1-11203). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GRASP55 Lysate (NBL1-11203) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for GRASP55 Lysate (NBL1-11203)

Discover related pathways, diseases and genes to GRASP55 Lysate (NBL1-11203). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for GRASP55 Lysate (NBL1-11203)

Discover more about diseases related to GRASP55 Lysate (NBL1-11203).
 

Pathways for GRASP55 Lysate (NBL1-11203)

View related products by pathway.

PTMs for GRASP55 Lysate (NBL1-11203)

Learn more about PTMs related to GRASP55 Lysate (NBL1-11203).
 

Research Areas for GRASP55 Lysate (NBL1-11203)

Find related products by research area.

Blogs on GRASP55

There are no specific blogs for GRASP55, but you can read our latest blog posts.

Customers Who Bought This Also Bought

GRASP55 Antibody
NBP1-89747

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our GRASP55 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol GORASP2