GRASP55 Antibody (CL2610) - Azide and BSA Free

Staining in U251 cell line with Anti-GRASP55 monoclonal antibody) .

Staining in U2OS cell line with Anti-GRASP55 monoclonal antibody) .

Staining in HeLa cell line with Anti-GRASP55 monoclonal antibody) .

Staining in A431 cell line with Anti-GRASP55 monoclonal antibody) .

Staining in MCF7 cell line with Anti-GRASP55 monoclonal antibody) .

Analysis in human epididymis and skeletal muscle tissues using NBP3-44478 antibody. Corresponding GRASP55 RNA-seq data are presented for the same tissues.

Staining of human epididymis shows strong golgi positivity in glandular cells.

Staining of human endometrium shows moderate to strong membranous and granular cytoplasmic positivity in glandular cells.

Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.

Staining of human prostate shows moderate to strong membranous and granular cytoplasmic positivity in glandular cells.

Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GRASP55 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL2610
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

GRASP55 Antibody (CL2610) - Azide and BSA Free Summary

• Immunogen: This antibody was generated using a recombinant protein sequence of Q9H8Y8, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: GORASP2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for GRASP55 Antibody (CL2610) - Azide and BSA Free

• Golgi phosphoprotein 6
• golgi reassembly stacking protein 2, 55 kDa
• golgi reassembly stacking protein 2, 55kDa
• Golgi reassembly-stacking protein of 55 kDa
• GOLPH6DKFZp434D156
• GRASP55FLJ13139
• GRS2Golgi reassembly-stacking protein 2
• p59

Background

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is involved in establishing the stacked structure of the Golgi apparatus. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GORASP2