GRAP Antibody

Western Blot: GRAP Antibody [NB100-800] - (0.3ug/ml) staining of human thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

GRAP Antibody Summary

• Immunogen: Peptide with sequence C-GFFPRSYVQPVHL corresponding to C-Terminus according to NP_006604.1.
• Predicted Species: Mouse (100%), Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GRAP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 26 kDa band observed in human thymus lysates (calculated MW of 25.3 kDa band according to NP_006604.1).
• Control:

  Jurkat Whole Cell Lysate
  GRAP Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GRAP Antibody

• GRB2-related adapter protein
• GRB2-related adaptor protein
• growth factor receptor-bound protein 2-related adaptor protein
• MGC64880

Background

A member of the GRB2/Sem5/Drk family containing an SH2 domain flanked by two SH3 domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GRAP Antibody (NB100-800)(7)

Publications using NB100-800

• Zampetidis CP, Galanos P, Angelopoulou A Et al. A recurrent chromosomal inversion suffices for driving escape from oncogene-induced senescence via subTAD reorganization Molecular cell 2021-11-12 [PMID: 34793711]
• Cottrell GS, Soubrane CH, Hounshell JA et al. CACHD1 is an a2d-like protein that modulates CaV3 voltage-gated calcium channel activity. J. Neurosci. 2018-09-04 [PMID: 30181139]
• Dehghan E, Zhang Y, Saremi B et al. Hydralazine induces stress resistance and extends C. elegans lifespan by activating the NRF2/SKN-1 signalling pathway. Nat Commun. 2017-12-20 [PMID: 29263362]
• Guo H, Kazadaeva Y, Ortega FE et al. Trinucleotide repeat containing 6c (TNRC6c) is essential for microvascular maturation during distal airspace sacculation in the developing lung Dev. Biol. 2017-08-12 [PMID: 28811219]
• Fathi A, Rasouli H, Yeganeh M et al. Efficient differentiation of human embryonic stem cells toward dopaminergic neurons using recombinant LMX1A factor. Mol Biotechnol 2015-02-01 [PMID: 25380985] (Human)
• McIntosh AL, Storey SM, Atshaves BP. Intracellular lipid droplets contain dynamic pools of sphingomyelin: ADRP binds phospholipids with high affinity. Lipids 2010-06-01 [PMID: 20473576]
• Feng GS, Ouyang YB, Hu DP et al. Grap is a novel SH3-SH2-SH3 adaptor protein that couples tyrosine kinases to the Ras pathway. J Biol Chem 1996-05-24 [PMID: 8647802]

Bioinformatics

• Gene Symbol: GRAP
• Entrez:
  • 10750(Human)
  • 71520(Mouse)
• Uniprot:
  • NP_006604.(Human)