Granzyme B Antibody [Biotin]

Granzyme B was detected in immersion fixed frozen sections of mouse spleen using Goat Anti-Mouse Granzyme B Biotinylated Antigen Affinity-purified Poly-clonal Antibody (Catalog # BAF1865) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Granzyme B was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse Granzyme B Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1865) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Mu
• Applications: WB, IHC, ELISA(Det), ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Granzyme B Antibody [Biotin] Summary

• Specificity: Detects mouse Granzyme B in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human (rh) Granzyme A, rhGranzyme B, recombinant mouse (rm) Granzyme D, rmGranzyme G, and rhGranzyme H is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GZMB
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Mouse Granzyme B Antibody (Catalog # AF1865) ELISA Detection: Mouse Granzyme B Biotinylated Antibody (Catalog # BAF1865) Standard: Recombinant Mouse Granzyme B (Catalog # 1865-SE)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Store the unopened product at -20 to -70 degreesC. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Granzyme B Antibody [Biotin]

• C11
• CCPI
• CGL1
• CGL-1
• CSPB
• CSP-B
• CTLA1
• CTLA-1
• CTSGL1
• Fragmentin-2
• Granzyme B
• Granzyme-2
• GranzymeB
• GRB
• GrzB
• GZMB
• HLP
• SECT

Background

Granzyme B is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1, 2). Granzyme B plays an essential role in granule-mediated apoptosis and may have additional roles in rheumatoid arthritis and in bacterial and viral infections (3). It activates various caspases and cleaves proteins such as aggrecan (3). Mouse Granzyme B is synthesized as a precursor (247 residues) with a signal and a pro peptide (residues 1-20) and a mature chain (residues 21-247) (4, 5). The recombinant mouse Granzyme B consisting of residues 17 to 247 was expressed and purified. After activation with cathepsin C, it cleaves a thioester substrate.

1. Kam, C.-M. et al. (2000) Biochim. Biophys. Acta 1477:307.
2. Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
3. Froelich, C.J. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 1549.
4. Lobe, C.G. et al. (1986) Science 232:858.
5. Brunet, J.F. et al. (1986) Nature 322:268.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Granzyme B Antibody (BAF1865)(11)

Publications using BAF1865

• Shingo Umemoto, Rika Nakahashi-Ouchida, Yoshikazu Yuki, Shiho Kurokawa, Tomonori Machita, Yohei Uchida et al. Cationic-nanogel nasal vaccine containing the ectodomain of RSV-small hydrophobic protein induces protective immunity in rodents npj Vaccines 7/24/2023 [PMID: 37488116]
• Yunjie Jin, Peng Gao, Lifei Liang, Yuhang Wang, Jiawei Li, Jiyan Wang et al. Noninvasive quantification of granzyme B in cardiac allograft rejection using targeted ultrasound imaging Frontiers in Immunology 6/26/2023 [PMID: 37435082]
• Yunjie Jin, Peng Gao, Lifei Liang, Yuhang Wang, Jiawei Li, Jiyan Wang et al. Noninvasive quantification of granzyme B in cardiac allograft rejection using targeted ultrasound imaging Frontiers in Immunology 2023-06-26 [PMID: 37435082] (Western Blot, In vivo assay, Mouse)
• YS Lim, AG Lee, X Jiang, JM Scott, A Cofie, S Kumar, D Kennedy, DJ Granville, H Shin NK cell-derived extracellular granzyme B drives epithelial ulceration during HSV-2 genital infection Cell Reports, 2023-04-17;42(4):112410. 2023-04-17 [PMID: 37071533] (IHC, Mouse)
• M Wroblewski, R Bauer, M Cubas Córd, F Udonta, I Ben-Batall, K Legler, C Hauser, J Egberts, M Janning, J Velthaus, C Schulze, K Pantel, C Bokemeyer, S Loges Mast cells decrease efficacy of anti-angiogenic therapy by secreting matrix-degrading granzyme B Nat Commun, 2017-08-16;8(1):269. 2017-08-16 [PMID: 28814715] (IHC, Mouse)
• MA Farrag, HM Amer, P Öhlschläge, ME Hamad, FN Almajhdi Novel Recombinant DNA Vaccine Candidates for Human Respiratory Syncytial Virus: Preclinical Evaluation of Immunogenicity and Protection Efficiency Hum Vaccin Immunother, 2017-03-08;0(0):0. 2017-03-08 [PMID: 28272978] (Flow Cytometry, Mouse)
• Fahad N. Almajhdi, Tilo Senger, Haitham M. Amer, Lutz Gissmann, Peter Öhlschläger Design of a Highly Effective Therapeutic HPV16 E6/E7-Specific DNA Vaccine: Optimization by Different Ways of Sequence Rearrangements (Shuffling) PLoS ONE 11/25/2014 [PMID: 25422946]
• Fahad N. Almajhdi, Tilo Senger, Haitham M. Amer, Lutz Gissmann, Peter Öhlschläger Design of a Highly Effective Therapeutic HPV16 E6/E7-Specific DNA Vaccine: Optimization by Different Ways of Sequence Rearrangements (Shuffling) PLoS ONE 2014-11-25 [PMID: 25422946] (Mouse)
• Whitehead M, Ohlschlager P, Almajhdi F, Alloza L, Marzabal P, Meyers A, Hitzeroth I, Rybicki E Human papillomavirus (HPV) type 16 E7 protein bodies cause tumour regression in mice. BMC Cancer, 2014-05-24;14(0):367. 2014-05-24 [PMID: 24885328] (ELISA Development (Detection), Mouse)
• Carnevalli L, Scognamiglio R, Cabezas-Wallscheid N, Rahmig S, Laurenti E, Masuda K, Jockel L, Kuck A, Sujer S, Polykratis A, Erlacher M, Pasparakis M, Essers M, Trumpp A Improved HSC reconstitution and protection from inflammatory stress and chemotherapy in mice lacking granzyme B. J Exp Med, 2014-04-21;211(5):769-79. 2014-04-21 [PMID: 24752302] (ICC, Mouse)
• Laurenti E, Varnum-Finney B, Wilson A, Ferrero I, Blanco-Bose WE, Ehninger A, Knoepfler PS, Cheng PF, MacDonald HR, Eisenman RN, Bernstein ID, Trumpp A Hematopoietic stem cell function and survival depend on c-Myc and N-Myc activity. Cell Stem Cell, 2008-12-04;3(6):611-24. 2008-12-04 [PMID: 19041778] (ICC, Mouse)

Bioinformatics

• Gene Symbol: GZMB
• Uniprot:
  • P04187()