Granzyme B Antibody (GrB-7) Summary
The antibody reacts with the 33 kD human serine protease granzyme B.
The monoclonal antibody reacts with the 33 kD human serine protease granzyme B. It does not react with human granzyme A. It is used as a marker for NK-cells and activated cytotoxic T-cells (CTL). This protease is localized in cytoplasmic granules and gives a granular staining pattern. Granzyme B is involved in target cell apoptosis during lymphocyte mediated cyto-toxicity. Exocytosis of granzyme containing granules in the cytoplasm of the target cell will lead to induction of DNA fragmentation and apoptosis of the target cell.
Tissue culture supernatant
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- Western Blot 1:100-1:2000
- Immunohistochemistry 1:10-1:500
- Immunohistochemistry-Frozen 1:10-1:500
- Immunohistochemistry-Paraffin 1:10-1:500
The monoclonal antibody stains granzyme B expressing lymphocytes in sections of sublimate formaldehyde as well as formalin fixed paraffin embedded tissues. It does not react with other cell types. The antibody can not be used on frozen sections. The antibBefore use in immunocytochemistry the slides have to be pretreated with 0.1 M sodium citrate, for 10 min. at 100 degrees C (in microwave oven). A working dilution of 1:20 is advised but optimal dilution should be tested by serial dilution.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
I ml tissue culture supernatant with 1.0% BSA
0.1% Sodium Azide
Tissue culture supernatant
Alternate Names for Granzyme B Antibody (GrB-7)
- Granzyme B
Granzyme B is a cysteine protease found in the cytoplasmic lysosome-like granules of cytolytic T lymphocytes (CTL) and natural killer (NK) cells and is is required for the induction of target cell lysis. This occurs as part of cell mediated immune responses, and can activate apoptosis in target cells by both caspase dependent and caspase independent mechanisms.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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