Granzyme B Antibody (216315)

• Reactivity: Mu
• Applications: WB
• Clone: 216315
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Granzyme B Antibody (216315) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Granzyme B
  Lys17-Ser247
  Accession # P04187
• Specificity: Detects mouse Granzyme B in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: GZMB
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Granzyme B Antibody (216315)

• C11
• CCPI
• CGL1
• CGL-1
• CSPB
• CSP-B
• CTLA1
• CTLA-1
• CTSGL1
• Fragmentin-2
• Granzyme B
• Granzyme-2
• GranzymeB
• GRB
• GrzB
• GZMB
• HLP
• SECT

Background

Granzyme B is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1, 2). Granzyme B plays an essential role in granule-mediated apoptosis and may have additional roles in rheumatoid arthritis and in bacterial and viral infections (3). It activates various caspases and cleaves proteins such as aggrecan (3). Mouse Granzyme B is synthesized as a precursor (247 residues) with a signal and a pro peptide (residues 1-20) and a mature chain (residues 21‑247) (4, 5). Mature mouse and human granzyme B share 69% amino acid sequence identity.

1. Kam, C.-M. et al. (2000) Biochim. Biophys. Acta 1477:307.
2. Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
3. Froelich, C.J. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 1549.
4. Lobe, C.G. et al. (1986) Science 232:858.
5. Brunet, J.F. et al. (1986) Nature 322:268.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Granzyme B Antibody (MAB1865)(1)

Publication using MAB1865

• Aubert, A;Liu, A;Kao, M;Goeres, J;Richardson, KC;Nierves, L;Jung, K;Nabai, L;Zhao, H;Orend, G;Krawetz, R;Lange, PF;Younger, A;Chan, J;Granville, DJ; Granzyme B cleaves Tenascin-C to release its C-terminal domain in rheumatoid arthritis JCI insight 2024-10-17 [PMID: 39475853] (Western Blot, Human)

Bioinformatics

• Gene Symbol: GZMB
• Uniprot:
  • P04187()