Granulin Antibody

Product Discontinued

Granulin Antibody Summary

• Immunogen: This antibody is specific for the Middle Region of the target protein.
• Specificity: This product is specific for Human grn.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GRN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Use in Blocking/Neutralizing reported in scientific literature (PMID: 24163244)

Reactivity Notes

Human. primate reactivity reported in scientific literature (PMID: 24163244)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.0) and 0.15M NaCl
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

Manufactured by SDIX's proprietary Genomic Antibody Technology™. GAT FAQs.

Alternate Names for Granulin Antibody

• acrogranin
• GEP
• GP88
• granulin
• granulin-epithelin
• granulins
• PC cell-derived growth factor
• PCDGF
• PEPI
• PGRN
• proepithelin
• progranulin

Background

Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Granulin Antibody (26320002)(4)

Publications using 26320002

• Holler CJ, Taylor G, Deng Q, Kukar T. Intracellular Proteolysis of Progranulin Generates Stable, Lysosomal Granulins that Are Haploinsufficient in Patients with Frontotemporal Dementia Caused by GRN Mutations eNeuro 2017-08-22 [PMID: 28828399] (WB, Human)
• Garrison C, Lastwika K, Zhang Y et al. Proteomic Analysis, Immune Dysregulation, and Pathway Interconnections With Obesity J Proteome Res. 2017-01-06 [PMID: 27769113] (MiAr)
  
  Details:
  Analysis is performed on plasma proteomic data to identify how obesity can alter pathways and to highlight the risk factor for disease in subjects with a high body mass index.
• Lee WC, Almeida S, Prudencio M et al. Targeted manipulation of the sortilin-progranulin axis rescues progranulin haploinsufficiency. Hum Mol Genet. 2013-11-07 [PMID: 24163244] (B/N, Primate)
• Rho JH, Lampe PD. High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays J Proteome Res. 2013-05-03 [PMID: 23541305] (MiAr)
  
  Details:
  A novel method using antibody microarrays is used to detect autoantibody-antigen complexes that can potentially be useful for detection and characterization of diseases.

FAQs for Granulin Antibody (26320002). (Showing 1 - 3 of 3 FAQs).

1. I'd like to get more information about this antibody, which is made by SDIX. We are using it for ICC and get a signal but it looks like it is in the nucleus. GRN hasn't been reported there so we are concerned about the specificity of this antibody. Can you tell me the target epitope region? Do you have sell the target peptide so we can do pre-absorption experiments? Have you tested it in ICC or ICC?
   • The epitope lies within the Granulin 4 region of the protein P28799 (https://www.uniprot.org/uniprotkb/P28799/entry) (a.a. 281 - 336). Unfortunately we do not sell the target protein or epitope. To my knowledge we do not have any ICC data to aid the client.
2. Does the antibody bind to a particular part of the granulin protein?
   • This is made to the middle region of the protein; however, we do not epitope map, so we do not know the specific binding site itself. Since it is polyclonal, it most likely binds to multiple regions.
3. Does the antibody detect splice variants of granulin?
   • This antibody targets the region of granulin that is cleaved to produce granulin 4. This region is present in all 3 splice isoforms listed in UNIPROT.

Bioinformatics

• Gene Symbol: GRN
• Entrez:
  • 2896(Human)
• Uniprot:
  • P28799(Human)