Mouse GR/NR3C1 ELISA Kit (Colorimetric)

ELISA: Mouse GR/NR3C1 ELISA Kit (Colorimetric) [NBP2-82191] - Samples were spiked with high concentrations of Mouse GR/NR3C1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse GR/NR3C1 ELISA Kit (Colorimetric) [NBP2-82191] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell supernatant
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.10 ng/mL

Mouse GR/NR3C1 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Mouse GR/NR3C1 in samples. No Significant cross-reactivity or interference between Mouse GR/NR3C1 and analogues was observed.
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.10 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5%
• Intra-Assay: CV% < 5%
• Spike Recovery: average = 100% for serum; average = 98% for EDTA plasma; average = 92% for cell culture media
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: NR3C1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse GR/NR3C1 ELISA Kit (Colorimetric)

• GCR
• glucocorticoid receptor
• GR
• GRGCCR
• GRLNuclear receptor subfamily 3 group C member 1
• NR3C1
• nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
• nuclear receptor subfamily 3, group C, member 1

Background

Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocoticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain glucocorticoid receptor. The corresponding gene for the glucocorticoid receptor is NR3C1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for GR/NR3C1 ELISA Kit (NBP2-82191). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: NR3C1