GR/NR3C1 Antibody (GT248) - Azide and BSA Free

Immunohistochemistry-Paraffin: GR/NR3C1 Antibody (GT248) [NBP3-13626] - GR/NR3C1 antibody [GT248] detects GR/NR3C1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. GR/NR3C1 stained by GR/NR3C1 antibody [GT248] (NBP3-13626) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: GR/NR3C1 Antibody (GT248) [NBP3-13626] - GR/NR3C1 antibody [GT248] detects GR/NR3C1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. GR/NR3C1 stained by GR/NR3C1 antibody [GT248] (NBP3-13626) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: GR/NR3C1 Antibody (GT248) [NBP3-13626] - GR/NR3C1 antibody [GT248] detects GR/NR3C1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. GR/NR3C1 stained by GR/NR3C1 antibody [GT248] (NBP3-13626) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Wild-type (WT) and NR3C1 knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glucocorticoid Receptor antibody [GT248] (NBP3-13626) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, IHC
• Clone: GT248
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

GR/NR3C1 Antibody (GT248) - Azide and BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human GR/NR3C1. The exact sequence is proprietary.
• Predicted Species: Bovine (90%). Backed by our 100% Guarantee.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: NR3C1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot

Reactivity Notes

Dog (88%), Cat (89%), Pig (83%), Sheep (89%), Guinea pig (85%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for GR/NR3C1 Antibody (GT248) - Azide and BSA Free

• GCR
• glucocorticoid receptor
• GR
• GRGCCR
• GRLNuclear receptor subfamily 3 group C member 1
• NR3C1
• nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
• nuclear receptor subfamily 3, group C, member 1

Background

Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocoticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain glucocorticoid receptor. The corresponding gene for the glucocorticoid receptor is NR3C1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NR3C1