![Western Blot: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] - Unboiled wild-type (WT) and GPRC5A knockout (KO) MCF-7 cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-212024939131.jpg "Western Blot: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] - Unboiled wild-type (WT) and GPRC5A knockout (KO) MCF-7 cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC |
| Clone | HL1864 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | Novus Biologicals Rabbit GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free (NBP3-25620) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant fragment of human GPRC5A/RAI3. |
| Source | HEK293 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | GPRC5A |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for GPRC5A/RAI3 Antibody (NBP3-25620)Find related products by research area.
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| Gene Symbol | GPRC5A |
![Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] - RAI3 antibody [HL1864] detects RAI3 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. RAI3 stained by RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-2120249432857.jpg "Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] - RAI3 antibody [HL1864] detects RAI3 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. RAI3 stained by RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] - Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-2120249342722.jpg "Western Blot: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] - Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![GPRC5A/RAI3 antibody [HL1864] detects GPRC5A/RAI3 protein by immunohistochemical analysis.Sample: Paraffin-embedded human tissues.GPRC5A/RAI3 stained by GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-immunohistochemistry-paraffin-141120251832019.jpg "GPRC5A/RAI3 antibody [HL1864] detects GPRC5A/RAI3 protein by immunohistochemical analysis.Sample: Paraffin-embedded human tissues.GPRC5A/RAI3 stained by GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![GPRC5A/RAI3 antibody [HL1864] detects GPRC5A/RAI3 protein by immunofluorescent analysis.Sample: Wild-type (WT) and GPRC5A/RAI3 knockout (KO) MCF-7 cells were fixed in ice-cold MeOH for 5 min.Green: GPRC5A/RAI3 stained by GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:500.Blue: Fluoroshield with DAPI](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-immunocytochemistry-immunofluorescence-1411202518404315.jpg "GPRC5A/RAI3 antibody [HL1864] detects GPRC5A/RAI3 protein by immunofluorescent analysis.Sample: Wild-type (WT) and GPRC5A/RAI3 knockout (KO) MCF-7 cells were fixed in ice-cold MeOH for 5 min.Green: GPRC5A/RAI3 stained by GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:500.Blue: Fluoroshield with DAPI")
![Non-transfected (-) and transfected (+) unboiled MCF-7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:5000 and competitor's antibody (Competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated withand does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-western-blot-1411202518442215.jpg "Non-transfected (-) and transfected (+) unboiled MCF-7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:5000 and competitor's antibody (Competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated withand does not endorse this product.")
![Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-western-blot-1411202518423013.jpg "Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Boiled and unboiled wild-type (WT) and GPRC5A/RAI3 knockout (KO) MCF-7 cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:5000 and competitor's antibody (Competitor) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated withand does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-western-blot-1411202518412910.jpg "Boiled and unboiled wild-type (WT) and GPRC5A/RAI3 knockout (KO) MCF-7 cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:5000 and competitor's antibody (Competitor) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated withand does not endorse this product.")
![GPRC5A/RAI3 antibody [HL1864] detects GPRC5A/RAI3 protein at cell membrane by immunofluorescent analysis.Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.Green: GPRC5A/RAI3 stained by GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-immunocytochemistry-immunofluorescence-1411202518335919.jpg "GPRC5A/RAI3 antibody [HL1864] detects GPRC5A/RAI3 protein at cell membrane by immunofluorescent analysis.Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.Green: GPRC5A/RAI3 stained by GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:500.Blue: Fluoroshield with DAPI .")
![GPRC5A/RAI3 antibody [HL1864] detects GPRC5A/RAI3 protein by immunohistochemical analysis.Sample: Paraffin-embedded human kidney.GPRC5A/RAI3 stained by GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:100 and highly cited competitor's antibody diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min*Competitor's antibody is not affiliated withand does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-immunohistochemistry-paraffin-141120251838289.jpg "GPRC5A/RAI3 antibody [HL1864] detects GPRC5A/RAI3 protein by immunohistochemical analysis.Sample: Paraffin-embedded human kidney.GPRC5A/RAI3 stained by GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:100 and highly cited competitor's antibody diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min*Competitor's antibody is not affiliated withand does not endorse this product.")
![Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-western-blot-1411202518404314.jpg "Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![GPRC5A/RAI3 antibody [HL1864] detects GPRC5A/RAI3 protein by immunohistochemical analysis.Sample: Paraffin-embedded human tissues.GPRC5A/RAI3 stained by GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-immunohistochemistry-paraffin-141120251840545.jpg "GPRC5A/RAI3 antibody [HL1864] detects GPRC5A/RAI3 protein by immunohistochemical analysis.Sample: Paraffin-embedded human tissues.GPRC5A/RAI3 stained by GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Unboiled non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-western-blot-141120251838173.jpg "Unboiled non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Flow Cytometry GPRC5C Antibody (577315) [Unconjugated]](https://images.novusbio.com/images2/GPRC5C_MAB6594_Flow_Cytometry_10478.jpg)
![Western Blot c-jun [p Ser63] Antibody (1018Y) [Unconjugated]](https://images.novusbio.com/images2/cJun_MAB8930_Western_Blot_18923.jpg)
![Immunocytochemistry c-jun [p Ser63] Antibody (1018Y) [Unconjugated]](https://images.novusbio.com/images2/cJun_MAB8930_Immunocytochemistry__Immunofluorescence_18945.jpg)
![Simple Western c-jun [p Ser63] Antibody (1018Y) [Unconjugated]](https://images.novusbio.com/images2/cJun_MAB8930_Simple_Western_19175.jpg)
![Western Blot Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Western_Blot_5287.jpg)
![Simple Western Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Simple_Western_16990.jpg)
![Knockout Validated Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Knockout_Validated_24032.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")