RAI3 Antibodies
We offer GPRC5A/RAI3 Antibodies for use in common research applications: ELISA, Flow (Intracellular), Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot. Each GPRC5A/RAI3 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our GPRC5A/RAI3 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the GPRC5A/RAI3 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our GPRC5A/RAI3 polyclonal antibodies and browse our GPRC5A/RAI3 monoclonal antibody catalog.
Alternate Names for RAI3 Antibodies
anti-GPRC5A/RAI3 antibody, anti-GPRC5A antibody, anti-G protein-coupled receptor, family C, group 5, member A antibody, anti-GPCR5A antibody, anti-Orphan G-protein-coupling receptor PEIG-1 antibody, anti-RAI3G-protein coupled receptor family C group 5 member A antibody, anti-RAIG1RAIG-1 antibody, anti-retinoic acid induced 3 antibody, anti-retinoic acid responsive antibody, anti-Retinoic acid-induced gene 1 protein antibody, anti-retinoic acid-induced protein 3 antibody
9 Results for "gprc5a-rai3" in Primary Antibodies
![Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody [NBP1-89743] - Analysis in human lung and skeletal muscle tissues. Corresponding GPRC5A RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GPRC5A-RAI3-Antibody-Immunohistochemistry-Paraffin-NBP1-89743-img0022.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody [NBP1-89743] - Analysis in human lung and skeletal muscle tissues. Corresponding GPRC5A RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: GPRC5A/RAI3 Antibody [NBP1-89743] - Western Blot analysis of different pathways were tested for alteration in knock-down with GPRC5A siRNA in comparison to negative control. Phosphorylation of STAT3 at Tyr705 is reduced in knock-down of all three cell lines, whereas Phospho-ERK is not changed significantly (see S2 Fig for original Western blot images). (n = 3). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0170390), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GPRC5A-RAI3-Antibody-Western-Blot-NBP1-89743-img0027.jpg)
![Immunocytochemistry/Immunofluorescence: GPRC5A/RAI3 Antibody [NBP1-89743] - Staining of human cell line U-2 OS shows localization to nucleoli, plasma membrane & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/GPRC5A-RAI3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89743-img0015.jpg)
![Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody [NBP1-89743] - Staining of human small intestine shows moderate positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/GPRC5A-RAI3-Antibody-Immunohistochemistry-Paraffin-NBP1-89743-img0026.jpg)
![Western Blot: GPRC5A/RAI3 Antibody [NBP1-89743] - (J) RAI3 has a conspicuously low expression in BxPc3 and PaCaDD165 in comparison to the other cell lines in the group. (n = 3). (PLoS One. 2017; 12 (1): e0170390. Published online 2017 Jan 23.)](http://images.novusbio.com/fullsize/GPRC5A-RAI3-Antibody-Western-Blot-NBP1-89743-img0012.jpg)
![Western Blot: GPRC5A/RAI3 Antibody [NBP1-89743] - Wester Blot: Pancreatic cell lines, where NC1 and NC2 represent negative controls.](http://images.novusbio.com/fullsize/GPRC5A-RAI3-Antibody-Western-Blot-NBP1-89743-img0014.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: GPRC5A/RAI3 Antibody [NBP1-89743] - Analysis in human cell lines A-431 and U-251MG using anti-GPRC5A antibody. Corresponding GPRC5A RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.](http://images.novusbio.com/fullsize/GPRC5A-RAI3-Antibody-Western-Blot-NBP1-89743-img0021.jpg)
![Immunohistochemistry Free-Floating: GPRC5A/RAI3 Antibody [NBP1-89743] - Expression of RAI3 in human tissue and cell lines. Immunochemistry with RAI3-Antibody in different types of pancreatic tissue. Presentation in 20-fold magnification of A-C: Normal; D: Chronic pancreatits (CP), E: Intraductal Papillary Mucinous Neoplasm (IPMN); F-H: Pancreatic Ductal Adenocarcinoma (PDAC). (PLoS One. 2017; 12 (1): e0170390. Published online 2017 Jan 23.)](http://images.novusbio.com/fullsize/GPRC5A-RAI3-Antibody-Immunohistochemistry-Free-Floating-NBP1-89743-img0013.jpg)
![Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody [NBP1-89743] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/GPRC5A-RAI3-Antibody-Immunohistochemistry-Paraffin-NBP1-89743-img0023.jpg)
![Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody [NBP1-89743] - Staining of human lung shows moderate membranous positivity in pneumocytes.](http://images.novusbio.com/fullsize/GPRC5A-RAI3-Antibody-Immunohistochemistry-Paraffin-NBP1-89743-img0024.jpg)
![Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody [NBP1-89743] - Staining of human urinary bladder shows moderate membranous positivity in urothelial cells.](http://images.novusbio.com/fullsize/GPRC5A-RAI3-Antibody-Immunohistochemistry-Paraffin-NBP1-89743-img0025.jpg)
![Western Blot: GPRC5A/RAI3 Antibody [NBP1-89743] - GPRC5a expression levels in normal pancreas & PaCa (PAAD) tissue & cell lines. (A,B) The data-mining analysis showed that GPRC5a was significantly upregulated in PaCa (PAAD) samples with an association of increased expression in higher malignant tumor stages (p < 0.05). (C,D) The Western blot & qPCR results showed that the basic protein-level expression of GPRC5a in PaCa cell lines was higher than that in the HPDEE6E7 pancreas cell line. (E) Immunofluorescence assay showed upregulated expression of GPRC5a in PaCa cell lines (200×). The error bars were from multiple samples (n) measurement results (A,B) or three experimental measurements (C,D). * p-value < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29949874), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-89743_rabbit-polyclonal-gprc5a-rai3-antibody-310202415384129.jpg)
![Western Blot: GPRC5A/RAI3 Antibody [NBP1-89743] - Influence of GPRC5a knockout on the proliferation & migration ability in MIA PaCa-2 & TB32047 cells. (A–D) Western blot & sequencing results showing the GPRC5a knocked out in MIA PaCa-2-KO1/2 & TB32047-KO1/2 (Red highlight area means the different part in comparison of sequencing sequence). (E,F): The knockout of GPRC5a inhibited the proliferation ability of MIA PaCa-2 & TB32047 cells. (G,H) GPRC5a knockout inhibited the migration ability of MIA PaCa-2 (G) & TB32047 cells (H). * p-value < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29949874), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-89743_rabbit-polyclonal-gprc5a-rai3-antibody-31020241517521.jpg)
![Western Blot: GPRC5A/RAI3 Antibody [NBP1-89743] - Influence of GPRC5a knockout on the proliferation & migration ability in MIA PaCa-2 & TB32047 cells. (A–D) Western blot & sequencing results showing the GPRC5a knocked out in MIA PaCa-2-KO1/2 & TB32047-KO1/2 (Red highlight area means the different part in comparison of sequencing sequence). (E,F): The knockout of GPRC5a inhibited the proliferation ability of MIA PaCa-2 & TB32047 cells. (G,H) GPRC5a knockout inhibited the migration ability of MIA PaCa-2 (G) & TB32047 cells (H). * p-value < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29949874), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-89743_rabbit-polyclonal-gprc5a-rai3-antibody-31020241530422.jpg)
![Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] - RAI3 antibody [HL1864] detects RAI3 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. RAI3 stained by RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-2120249432857.jpg "Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] - RAI3 antibody [HL1864] detects RAI3 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. RAI3 stained by RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![<b>Genetic Strategies Validation. </b>Western Blot: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] - Unboiled wild-type (WT) and GPRC5A knockout (KO) MCF-7 cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-212024939131.jpg)
![Western Blot: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-2120249422659.jpg)
![Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] - RAI3 antibody [HL1864] detects RAI3 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human kidney. RAI3 stained by RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-2120249435510.jpg)
![Western Blot: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] - Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-2120249342722.jpg)
![Western Blot: GPRC5A/RAI3 Antibody [H00009052-B01P] - Analysis of GPRC5A expression in human ovarian cancer.](http://images.novusbio.com/fullsize/GPRC5A-RAI3-Antibody-Western-Blot-H00009052-B01P-img0001.jpg "Western Blot: GPRC5A/RAI3 Antibody [H00009052-B01P] - Analysis of GPRC5A expression in human ovarian cancer.")
![Western Blot: GPRC5A/RAI3 Antibody [H00009052-B01P] - Analysis of GPRC5A expression in transfected 293T cell line by GPRC5A polyclonal antibody. Lane 1: GPRC5A transfected lysate(39.27 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/GPRC5A-RAI3-Antibody-Western-Blot-H00009052-B01P-img0002.jpg)
![Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody (GPRC5A/7893) [NBP3-23915] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with GPRC5A/RAI3 Antibody (GPRC5A/7893). Inset: PBS instead of primary antibody; secondary only negative control.](http://images.novusbio.com/fullsize/nbp3-23915_mouse-gprc5a-rai3-mab-gprc5a-7893-2412025863688.jpg "Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody (GPRC5A/7893) [NBP3-23915] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with GPRC5A/RAI3 Antibody (GPRC5A/7893). Inset: PBS instead of primary antibody; secondary only negative control.")
![Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody (GPRC5A/7893) [NBP3-23915] - Formalin-fixed, paraffin-embedded human bladder stained with GPRC5A/RAI3 Antibody (GPRC5A/7893). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/nbp3-23915_mouse-gprc5a-rai3-mab-gprc5a-7893-2412025872358.jpg)
![Immunoprecipitation:[NBP3-30588]-Detection of human GPRC5A by western blot of immunoprecipitates. Samples: Whole cell lysate prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit antibody used for IP at 6 µg per reaction. GPRC5A was also immunoprecipitated by rabbit antibody. For blotting immunoprecipitated GPRC5A, was used at 0. 4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-30588_rabbit-gprc5a-rai3-pab-231202511585558.jpg "Immunoprecipitation:[NBP3-30588]-Detection of human GPRC5A by western blot of immunoprecipitates. Samples: Whole cell lysate prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit antibody used for IP at 6 µg per reaction. GPRC5A was also immunoprecipitated by rabbit antibody. For blotting immunoprecipitated GPRC5A, was used at 0. 4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunocytochemistry/Immunofluorescence: GPRC5A/RAI3 Antibody [NBP2-58188] - Staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.](http://images.novusbio.com/fullsize/GPRC5A-RAI3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58188-img0001.jpg "Immunocytochemistry/Immunofluorescence: GPRC5A/RAI3 Antibody [NBP2-58188] - Staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.")
![Immunoprecipitation:GPRC5A/RAI3 AntibodyNBP3-30195] -Detection of human GPRC5A by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-GPRC5A antibody NBP3-30195 (lot NBP3-30195-1) used for IP at 6 µg per reaction. GPRC5A was also immunoprecipitated by rabbit anti-GPRC5A.. For blotting immunoprecipitated GPRC5A, NBP3-30195 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-30195_rabbit-gprc5a-rai3-pab-3012202416502223.jpg "Immunoprecipitation:GPRC5A/RAI3 AntibodyNBP3-30195] -Detection of human GPRC5A by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-GPRC5A antibody NBP3-30195 (lot NBP3-30195-1) used for IP at 6 µg per reaction. GPRC5A was also immunoprecipitated by rabbit anti-GPRC5A.. For blotting immunoprecipitated GPRC5A, NBP3-30195 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")