GPRC5C Antibody (577315) [Unconjugated]

PC‑3 human prostate cancer cell line was stained with Human GPRC5C Monoclonal Antibody (Catalog # MAB6594, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 577315
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

GPRC5C Antibody (577315) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with recombinant human GPRC5C
  Accession # Q9NQ84
• Specificity: Detects human GPRC5C in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: GPRC5C
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GPRC5C Antibody (577315) [Unconjugated]

• G protein-coupled receptor, family C, group 5, member C
• GPRC5C
• MGC131820
• RAIG3
• RAIG-3G-protein coupled receptor family C group 5 member C
• RAIG3orphan G-protein coupled receptor
• retinoic acid responsive gene protein
• Retinoic acid-induced gene 3 protein

Background

G-protein coupled receptor family C group 5 member C (GPRC5C), also known as Retinoic acid induced gene 3 (RAIG-3), is an approximately 50 kDa protein with seven transmembrane segments and one site of potential N-linked glycosylation. It is predominantly expressed in the stomach, kidney, liver, pancreas, and prostate. Human GPRC5C shares 88% and 90% amino acid sequence identity with mouse and rat GPRC5C, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GPRC5C Antibody (MAB6594)(2)

Publications using MAB6594

• YW Zhang, J Mess, N Aizarani, P Mishra, C Johnson, MC Romero-Mul, J Rettkowski, K Schönberge, N Obier, K Jäcklein, NM Woessner, ME Lalioti, T Velasco-He, K Sikora, R Wäsch, B Lehnertz, G Sauvageau, T Manke, P Menendez, SG Walter, S Minguet, E Laurenti, S Günther, D Grün, N Cabezas-Wa Hyaluronic acid-GPRC5C signalling promotes dormancy in haematopoietic stem cells Nature Cell Biology, 2022-06-20;0(0):. 2022-06-20 [PMID: 35725769] (Proximity Ligation Assay, Human)
• Guan-Da Syu, Shih-Chin Wang, Guangzhong Ma, Shuang Liu, Donna Pearce, Atish Prakash et al. Development and application of a high-content virion display human GPCR array Nature Communications 4/30/2019 [PMID: 31040288]

Bioinformatics

• Gene Symbol: GPRC5C
• Uniprot:
  • Q9NQ84()