| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89743-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB5239-SP | |||
| Carrier Free | IC5239R-100UG | |||
| Carrier Free | IC5239N-100UG | |||
| Carrier Free | IC5239V-100UG | |||
| Carrier Free | IC5239G-100UG | |||
| Carrier Free | IC5239U-100UG | |||
| Carrier Free | IC5239T-100UG | |||
| Carrier Free | IC5239S-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-58188-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-24315 | |||
![Immunocytochemistry/Immunofluorescence: GPRC5A/RAI3 Antibody [NBP1-89743] - Staining of human cell line U-2 OS shows localization to nucleoli, plasma membrane & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/GPRC5A-RAI3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89743-img0015.jpg "Immunocytochemistry/Immunofluorescence: GPRC5A/RAI3 Antibody [NBP1-89743] - Staining of human cell line U-2 OS shows localization to nucleoli, plasma membrane & vesicles. Antibody staining is shown in green.")
![Immunohistochemistry Free-Floating: GPRC5A/RAI3 Antibody [NBP1-89743] - Expression of RAI3 in human tissue and cell lines. Immunochemistry with RAI3-Antibody in different types of pancreatic tissue. Presentation in 20-fold magnification of A-C: Normal; D: Chronic pancreatits (CP), E: Intraductal Papillary Mucinous Neoplasm (IPMN); F-H: Pancreatic Ductal Adenocarcinoma (PDAC). (PLoS One. 2017; 12 (1): e0170390. Published online 2017 Jan 23.)](http://images.novusbio.com/fullsize/GPRC5A-RAI3-Antibody-Immunohistochemistry-Free-Floating-NBP1-89743-img0013.jpg)
![Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody [NBP1-89743] - Staining of human lung shows moderate membranous positivity in pneumocytes.](http://images.novusbio.com/fullsize/GPRC5A-RAI3-Antibody-Immunohistochemistry-Paraffin-NBP1-89743-img0024.jpg)
![Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody [NBP1-89743] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/GPRC5A-RAI3-Antibody-Immunohistochemistry-Paraffin-NBP1-89743-img0023.jpg)
![Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody [NBP1-89743] - Staining of human small intestine shows moderate positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/GPRC5A-RAI3-Antibody-Immunohistochemistry-Paraffin-NBP1-89743-img0026.jpg)
![Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody [NBP1-89743] - Staining of human urinary bladder shows moderate membranous positivity in urothelial cells.](http://images.novusbio.com/fullsize/GPRC5A-RAI3-Antibody-Immunohistochemistry-Paraffin-NBP1-89743-img0025.jpg)
![Western Blot: GPRC5A/RAI3 Antibody [NBP1-89743] - (J) RAI3 has a conspicuously low expression in BxPc3 and PaCaDD165 in comparison to the other cell lines in the group. (n = 3). (PLoS One. 2017; 12 (1): e0170390. Published online 2017 Jan 23.)](http://images.novusbio.com/fullsize/GPRC5A-RAI3-Antibody-Western-Blot-NBP1-89743-img0012.jpg)
![Western Blot: GPRC5A/RAI3 Antibody [NBP1-89743] - Wester Blot: Pancreatic cell lines, where NC1 and NC2 represent negative controls.](http://images.novusbio.com/fullsize/GPRC5A-RAI3-Antibody-Western-Blot-NBP1-89743-img0014.jpg)
![Western Blot: GPRC5A/RAI3 Antibody [NBP1-89743] - GPRC5a expression levels in normal pancreas & PaCa (PAAD) tissue & cell lines. (A,B) The data-mining analysis showed that GPRC5a was significantly upregulated in PaCa (PAAD) samples with an association of increased expression in higher malignant tumor stages (p < 0.05). (C,D) The Western blot & qPCR results showed that the basic protein-level expression of GPRC5a in PaCa cell lines was higher than that in the HPDEE6E7 pancreas cell line. (E) Immunofluorescence assay showed upregulated expression of GPRC5a in PaCa cell lines (200×). The error bars were from multiple samples (n) measurement results (A,B) or three experimental measurements (C,D). * p-value < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29949874), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-89743_rabbit-polyclonal-gprc5a-rai3-antibody-310202415384129.jpg)
![Western Blot: GPRC5A/RAI3 Antibody [NBP1-89743] - Influence of GPRC5a knockout on the proliferation & migration ability in MIA PaCa-2 & TB32047 cells. (A–D) Western blot & sequencing results showing the GPRC5a knocked out in MIA PaCa-2-KO1/2 & TB32047-KO1/2 (Red highlight area means the different part in comparison of sequencing sequence). (E,F): The knockout of GPRC5a inhibited the proliferation ability of MIA PaCa-2 & TB32047 cells. (G,H) GPRC5a knockout inhibited the migration ability of MIA PaCa-2 (G) & TB32047 cells (H). * p-value < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29949874), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-89743_rabbit-polyclonal-gprc5a-rai3-antibody-31020241530422.jpg)
![Western Blot: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] - Unboiled wild-type (WT) and GPRC5A knockout (KO) MCF-7 cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-212024939131.jpg "<b>Genetic Strategies Validation. </b>Western Blot: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] - Unboiled wild-type (WT) and GPRC5A knockout (KO) MCF-7 cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] - RAI3 antibody [HL1864] detects RAI3 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. RAI3 stained by RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-2120249432857.jpg)
![Western Blot: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] - Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-2120249342722.jpg)
![<b>Orthogonal Strategies Validation. </b>GPRC5A/RAI3 antibody [HL1864] detects GPRC5A/RAI3 protein by immunohistochemical analysis.Sample: Paraffin-embedded human tissues.GPRC5A/RAI3 stained by GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-immunohistochemistry-paraffin-141120251832019.jpg)
![<b>Genetic Strategies Validation. </b>GPRC5A/RAI3 antibody [HL1864] detects GPRC5A/RAI3 protein by immunofluorescent analysis.Sample: Wild-type (WT) and GPRC5A/RAI3 knockout (KO) MCF-7 cells were fixed in ice-cold MeOH for 5 min.Green: GPRC5A/RAI3 stained by GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:500.Blue: Fluoroshield with DAPI](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-immunocytochemistry-immunofluorescence-1411202518404315.jpg)
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Non-transfected (-) and transfected (+) unboiled MCF-7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:5000 and competitor's antibody (Competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated withand does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-western-blot-1411202518442215.jpg)
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-western-blot-1411202518423013.jpg)
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Boiled and unboiled wild-type (WT) and GPRC5A/RAI3 knockout (KO) MCF-7 cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:5000 and competitor's antibody (Competitor) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated withand does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-western-blot-1411202518412910.jpg)
![GPRC5A/RAI3 antibody [HL1864] detects GPRC5A/RAI3 protein at cell membrane by immunofluorescent analysis.Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.Green: GPRC5A/RAI3 stained by GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-immunocytochemistry-immunofluorescence-1411202518335919.jpg)
![<b>Independent Antibodies Validation. </b>GPRC5A/RAI3 antibody [HL1864] detects GPRC5A/RAI3 protein by immunohistochemical analysis.Sample: Paraffin-embedded human kidney.GPRC5A/RAI3 stained by GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:100 and highly cited competitor's antibody diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min*Competitor's antibody is not affiliated withand does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-immunohistochemistry-paraffin-141120251838289.jpg)
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-western-blot-1411202518404314.jpg)
![<b>Orthogonal Strategies Validation. </b>GPRC5A/RAI3 antibody [HL1864] detects GPRC5A/RAI3 protein by immunohistochemical analysis.Sample: Paraffin-embedded human tissues.GPRC5A/RAI3 stained by GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-immunohistochemistry-paraffin-141120251840545.jpg)
![<b>Expression of Tagged Proteins Validation. </b>Unboiled non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-western-blot-141120251838173.jpg)
![Western Blot: GPRC5A/RAI3 Antibody [H00009052-B01P] - Analysis of GPRC5A expression in human ovarian cancer.](http://images.novusbio.com/fullsize/GPRC5A-RAI3-Antibody-Western-Blot-H00009052-B01P-img0001.jpg "Western Blot: GPRC5A/RAI3 Antibody [H00009052-B01P] - Analysis of GPRC5A expression in human ovarian cancer.")
![Western Blot: GPRC5A/RAI3 Antibody [H00009052-B01P] - Analysis of GPRC5A expression in transfected 293T cell line by GPRC5A polyclonal antibody. Lane 1: GPRC5A transfected lysate(39.27 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/GPRC5A-RAI3-Antibody-Western-Blot-H00009052-B01P-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: GPRC5A/RAI3 Antibody [NBP2-58188] - Staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.](http://images.novusbio.com/fullsize/GPRC5A-RAI3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58188-img0001.jpg "Immunocytochemistry/Immunofluorescence: GPRC5A/RAI3 Antibody [NBP2-58188] - Staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.")
![Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody (GPRC5A/7893) [NBP3-23915] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with GPRC5A/RAI3 Antibody (GPRC5A/7893). Inset: PBS instead of primary antibody; secondary only negative control.](http://images.novusbio.com/fullsize/nbp3-23915_mouse-gprc5a-rai3-mab-gprc5a-7893-2412025863688.jpg "Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody (GPRC5A/7893) [NBP3-23915] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with GPRC5A/RAI3 Antibody (GPRC5A/7893). Inset: PBS instead of primary antibody; secondary only negative control.")
![Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody (GPRC5A/7893) [NBP3-23915] - Formalin-fixed, paraffin-embedded human bladder stained with GPRC5A/RAI3 Antibody (GPRC5A/7893). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/nbp3-23915_mouse-gprc5a-rai3-mab-gprc5a-7893-2412025872358.jpg)
![Immunoprecipitation:[NBP3-30588]-Detection of human GPRC5A by western blot of immunoprecipitates. Samples: Whole cell lysate prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit antibody used for IP at 6 µg per reaction. GPRC5A was also immunoprecipitated by rabbit antibody. For blotting immunoprecipitated GPRC5A, was used at 0. 4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-30588_rabbit-gprc5a-rai3-pab-231202511585558.jpg "Immunoprecipitation:[NBP3-30588]-Detection of human GPRC5A by western blot of immunoprecipitates. Samples: Whole cell lysate prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit antibody used for IP at 6 µg per reaction. GPRC5A was also immunoprecipitated by rabbit antibody. For blotting immunoprecipitated GPRC5A, was used at 0. 4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: RAI3 Overexpression Lysate (Adult Normal) [NBL1-11303] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RAI3.](http://images.novusbio.com/fullsize/RAI3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11303-img0002.jpg "Western Blot: RAI3 Overexpression Lysate (Adult Normal) [NBL1-11303] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RAI3.")
![Immunoprecipitation:GPRC5A/RAI3 AntibodyNBP3-30195] -Detection of human GPRC5A by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-GPRC5A antibody NBP3-30195 (lot NBP3-30195-1) used for IP at 6 µg per reaction. GPRC5A was also immunoprecipitated by rabbit anti-GPRC5A.. For blotting immunoprecipitated GPRC5A, NBP3-30195 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-30195_rabbit-gprc5a-rai3-pab-3012202416502223.jpg "Immunoprecipitation:GPRC5A/RAI3 AntibodyNBP3-30195] -Detection of human GPRC5A by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-GPRC5A antibody NBP3-30195 (lot NBP3-30195-1) used for IP at 6 µg per reaction. GPRC5A was also immunoprecipitated by rabbit anti-GPRC5A.. For blotting immunoprecipitated GPRC5A, NBP3-30195 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![ELISA: Human GPRC5A/RAI3 ELISA Kit (Colorimetric) [NBP3-31735] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31735_human-gprc5a-rai3-elisa-kit-colorimetric-206202415301538.png "ELISA: Human GPRC5A/RAI3 ELISA Kit (Colorimetric) [NBP3-31735] - Standard Curve Reference")
![ELISA: Human GPRC5A/RAI3 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31736] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31736_human-gprc5a-rai3-ready-to-use-elisa-kit-colorimetric-206202415314879.png "ELISA: Human GPRC5A/RAI3 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31736] - Standard Curve Reference")