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F0101B). Quadrant markers were set based on isotype control antibody (MAB003). Staining was performed using our Staining Membrane-Associated Proteins protocol. " class="big_lightbox" target="_blank">
MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_lightbox" target="_blank">