GPR37L1 Antibody (444532) [Unconjugated]

A172 human glioblastoma cell line was stained with Mouse Anti-Human GPR37L1 Monoclonal Antibody (Catalog # MAB4449, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

• Reactivity: Hu
• Applications: CyTOF-ready, Flow
• Clone: 444532
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

GPR37L1 Antibody (444532) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human GPR37L1
  Accession # O60883
• Specificity: Detects human GPR37L1. Stains human GPR37L1 transfectants but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: GPR37L1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GPR37L1 Antibody (444532) [Unconjugated]

• endothelin B receptor-like protein 2
• ET(B)R-LP-2
• ETBRLP2
• ETBR-LP-2
• ETBR-LP-2endothelin type b receptor-like protein 2
• G protein-coupled receptor 37 like 1
• GPR37L1
• G-protein coupled receptor 37 like 1
• G-protein coupled receptor 37-like 1

Background

GPR37L1 (G-protein coupled receptor 37-like 1), also called ETBR-LP-2 (endothelin B receptor-like protein 2), is a 481 aa, orphan 7-transmembrane receptor with calculated molecular weight of 53 kDa. GPR37L1 is expressed in cerebellar Bergmann glia, cerebral cortex and internal capsule fibers in the central nervous system. It shows 39% aa identity with GPR37, a neuronal protein that accumulates in juvenile Parkinson’s disease neurons under endoplasmic reticulum stress. GPR37L1 does not bind known endothelin B family members. The extracellular portions of human and mouse GPR37L1 share 85% aa identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GPR37L1
• Uniprot:
  • O60883()