GPR155 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: GPR155 Antibody [NBP1-50625] - U-87 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-GPR155 Antibody NBP1-50625 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunohistochemistry-Paraffin: GPR155 Antibody [NBP1-50625] - Analysis of a FFPE tissue section of human kidney using 1:200 dilution of GPR155 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.06 mg/ml

GPR155 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit GPR155 Antibody - BSA Free (NBP1-50625) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide made to an internal portion of the human GPR155 protein (between residues 500-600) [UniProt Q7Z3F1]
• Localization: Membrane; Multi-pass membrane protein.Mem
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GPR155
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 1:100-1:2000
• Application Notes: This GPR155 antibody has been tested with inconclusive results in Western blot and Immunofluorescence.

Reactivity Notes

Immunogen sequence has 86% identity to mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1.06 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GPR155 Antibody - BSA Free

• DEP.7
• DEPDC3
• FLJ31819
• FLJ39346
• G protein-coupled receptor 155
• integral membrane protein GPR155
• PGR22G-protein coupled receptor PGR22

Background

GPR155 (G-protein coupled receptor PGR22) is an integral membrane protein identified through systematic search for homologies to known GPCRs and has been suggested to be implicated in Huntington disease and autism spectrum disorders. In mouse model, GPR155 mRNAs have been shown to be widely distributed in forebrain regions with more restricted distribution in the mid/hindbrain and highest levels in lateral parts of hippocampus as well as striatum, and its expression pattern in striatum resembled cannabinoid receptor type 1. GPR155 protein has been proposed to implicate in the sensory information processing and memory because of its high expression in cerebral cortex, hippocampus, septal nuclei, amygdaloid complex and that it is significantly dysregulated in autism spectrum disorders which are characterized by impaired social interaction/communication, restricted and repetitive interests and activities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GPR155
• Entrez:
  • 151556(Human)
  • 68526(Mouse)
• Uniprot:
  • Q7Z3F1(Human)
  • A2AWR3(Mouse)