GPR119 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: GPR119 Antibody [NLS548] - GPR119 antibody was tested in MCF-7 cells with Dylight 488 (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: GPR119 Antibody [NLS548] - Staining of human brain, neuron.
Immunohistochemistry: GPR119 Antibody [NLS548] - Analysis of GPR119 in mouse pancreas using DAB with hematoxylin counterstain.
Immunohistochemistry: GPR119 Antibody [NLS548] - Analysis of GPR119 in human pancreas using Fast Red with hematoxylin counterstain.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

GPR119 Antibody Summary

Immunogen
A synthetic peptide made to an internal region of the human GPR119 protein (within residues 150-250). [Swiss-Prot Q8TDV5]
Localization
Cell membrane; Multi-pass membrane protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GPR119
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
Application Notes
This GPR119 antibody is useful for Immunohistochemistry paraffin embedded sections and Immunocytochemistry/Immunofluorescence. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Positive Control
GPR119 Lysate (NBL1-11245)

Reactivity Notes

Human and mouse. Immunogen sequence has 88% identity to rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GPR119 Antibody

  • G protein-coupled receptor 119
  • glucose-dependent insulinotropic receptor
  • GPCR2
  • GPR119
  • G-protein coupled receptor 119
  • G-protein coupled receptor 2
  • hGPCR2
  • MGC119957

Background

GPR119 (G-protein coupled receptor 119) is a class A (rhodopsin-like) 7-transmembrane GPCR with little homology to other receptors, and human GPR119 receptor has 335 amino acids which is highly conserved in mouse and rat. GPR119 is expressed in human insulin-secreting pancreatic islets and incretin (GIP and GLP-1) secreting K- and L-cells of gastrointestinal tract. GPR119 has been suggested to function as a glucose-dependent insulinotropic receptor and its activity is mediated by G proteins which activate adenylate cyclase. Lysophosphatidylcholine (LPC), oleoylethanolamide (OEA), N-acylated ethanolamine phospholipids (NAPEs) and N-oleoyldopamine (OLDA), and 5-hydroxyeicosapentaenoic acid (5-HEPE) have all been proposed as GPR119's endogenous ligands. Moreover, GPR119 agonists have been shown to elicit nutrient-independent incretin and gastrointestinal hormone release as well as nutrient-stimulated insulin secretion, thereby lowering plasma glucose levels during an oral glucose tolerance test. In rodent models, GPR119 mediates improved glucose tolerance and prevents weight gain. GPR119 agonism increases the release of incretin hormones and insulin from enteroendocrine and pancreatic beta cells which may underlie these beneficial effects. GPR119 agonists play a role in reducing food intake and promoting weight loss, possibly through the release of GLP-1, which is known to delay gastric emptying.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics

Gene Symbol GPR119
Entrez
Uniprot