FFAR4/GPR120 Antibody

Images

 
Western Blot: FFAR4/GPR120 Antibody [NBP1-00858] - Analysis of GPR120 (V259) antibody in extracts from LOVO cells.
Immunocytochemistry/ Immunofluorescence: FFAR4/GPR120 Antibody [NBP1-00858] - GPR120 antibody was tested at 1:50 in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Immunohistochemistry-Paraffin: FFAR4/GPR120 Antibody [NBP1-00858] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse intestine using FFAR4/GPR120 antibody at 1:200 dilution. The intestinal ...read more
Immunohistochemistry-Paraffin: FFAR4/GPR120 Antibody [NBP1-00858] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse intestine using FFAR4/GPR120 antibody at 1:200 dilution. The intestinal ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

FFAR4/GPR120 Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of the human GPR120 protein (between residues 200-300) [UniProt Q5NUL3]
Localization
FFAR4/GPR120 is a transmembrane protein with extracellular-helical-cytoplasmic domains
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FFAR4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
Reviewed Applications
Read 1 Review rated 5
using
NBP1-00858 in the following applications:

Publications
Read Publications using
NBP1-00858 in the following applications:

  • 2 publications
  • IHC
    1 publication
  • WB
    6 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FFAR4/GPR120 Antibody

  • FFAR4
  • G protein-coupled receptor 120
  • G protein-coupled receptor 129
  • G protein-coupled receptor PGR4
  • GPR120
  • GPR129
  • G-protein coupled receptor 120
  • G-protein coupled receptor 129
  • G-protein coupled receptor GT01
  • G-protein coupled receptor PGR4
  • G-protein-coupled receptor GT01
  • GT01
  • MGC119984
  • O3FAR1
  • omega-3 fatty acid receptor 1
  • PGR4
  • PGR4DKFZp686F0824

Background

GPR120, a member of the rhodopsin family of G protein-coupled receptors (GPCRs), is a 377 amino acid protein which is expressed in the intestine. GPR120 is a receptor for unsaturated long-chain FFAs (free fatty acids). FFAs act as signaling molecules and are an important energy source. They also employ various physiological responses through their GPCRs. One such response occurs when dietary FFAs stimulate GPR120. This stimulation promotes the secretion of Glucagon-like peptide-1 (GLP-1) in vivo and in vitro.GLP-1 belongs to the class of molecules known as the incretins, which are associated with insulin secreted from the pancreas as a result of food intake.GLP-1 also inhibits Glucagon and gastric acid secretion and gastric emptying. Consequently, the role of GPR120 in the secretion of GLP-1 is critical in the treatment of diabetes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-1537
Species: Hu
Applications: WB, PEP-ELISA
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NLS6599
Species: Hu, Mk, Pm
Applications: IHC-P
NBP1-35641
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-14014
Species: Hu
Applications: IHC, IHC-P
NLS548
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
NB400-144
Species: Hu, Mu, Rt, Po, Bv, Pm
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
MAB8864
Species: Hu
Applications: IHC
NLS343
Species: Hu, Eq, Mk
Applications: IHC, IHC-P
H00008767-M01
Species: Hu, Mu, Rt
Applications: WB, ELISA
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NLS418
Species: Hu
Applications: IHC-P
NBP2-22106
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NB100-1533
Species: Hu, Mu, Po, Sh
Applications: WB, ICC/IF, IHC, IHC-P, PEP-ELISA

Publications for FFAR4/GPR120 Antibody (NBP1-00858)(12)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


Filter By Application
ICC/IF
(2)
IHC
(1)
WB
(6)
All Applications
Filter By Species
Human
(3)
Mouse
(6)
Rat
(3)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NBP1-00858 Applications Species
Zhu S, Jiang X, Jiang S et al. GPR120 is not required for w-3 PUFAs-induced cell growth inhibition and apoptosis in breast cancer cells. Cell Biol. Int. 2017 Oct 05 [PMID: 28980737] (Human) Human
Cheshmehkani A, Senatorov IS, Dhuguru J et al. Free-fatty acid receptor-4 (FFA4) modulates ROS generation and COX-2 expression via the C-terminal B-arrestin phosphosensor in Raw 264.7 macrophages Biochem. Pharmacol. 2017 Sep 21 [PMID: 28943238] (Mouse) Mouse
Lee KP, Park SJ, Kang S et al. Omega-3 polyunsaturated fatty acids accelerate airway repair by activating FFA4 in club cells. Am. J. Physiol. Lung Cell Mol. Physiol. Mar 17 2017 [PMID: 28314803] (WB, Mouse) WB Mouse
Houthuijzen JM, Oosterom I, Hudson BD et al. Fatty acid 16:4(n-3) stimulates a GPR120-induced signaling cascade in splenic macrophages to promote chemotherapy resistance. FASEB J. 2017 Feb 09 [PMID: 28183801]
Zhang D, So WY, Wang Y et al. Insulinotropic effects of GPR120 agonists are altered in obese non-diabetic and diabetic states. Clin. Sci. Dec 15 2016 [PMID: 27980130] (Rat) Rat
Baron M, Veres A, Wolock SL et al. A Single-Cell Transcriptomic Map of the Human and Mouse Pancreas Reveals Inter- and Intra-cell Population Structure. Cell Syst Oct 26 2016 [PMID: 27667365] (IHC, Mouse) IHC Mouse
Lee J, Moraes-Vieira Pm, Castoldi A et al. Branched fatty acid esters of hydroxy fatty acids (FAHFAs) protect against colitis by regulating the gut innate and adaptive immune systems. J. Biol. Chem. Aug 29 2016 [PMID: 27573241] (WB) WB
Anbazhagan AN, Priyamvada S, Gujral T et al. A Novel Anti-inflammatory Role of GPR120 in Intestinal Epithelial Cells. Am. J. Physiol., Cell Physiol. 2016 Jan 20 [PMID: 26791484] (WB, ICC/IF, Human, Mouse, Rat)

Details:
GPR120 antibody was used at 1:75 dilution in immunofluorescent / ICC-IF staining of control or agonist-treated (GW9508 or TUG-891, 6 hr) STC-1 cells grown on 6-well transwell inserts. The antibody was used for WB also on lysates from human, mouse or rat's intestinal mucosal tissues or from Caco-2 and STC-1 cell lines grown under several different experimental conditions.
WB, ICC/IF Human, Mouse, Rat
Kim JM, Lee KP, Park SJ et al. Omega-3 fatty acids induce Ca(2+) mobilization responses in human colon epithelial cell lines endogenously expressing FFA4. Acta Pharmacol. Sin. 2015 May 25 [PMID: 26005911] (ICC/IF, Human)

Details:
FFAR4/GPR120 antibody was used at 1:500 dilution in ICC-IF immunostaining assay for the detection of endogenous free fatty acid receptor 4 (FFA4) protein in HCT116 and HT-29 human colon cancer cells 30 min after exposing the cells to 50uM of alpha linolenic acid/LA, eicosapentaenoic acid / ELA or docosahexaenoic acid /DHA (Figure 1C and 1D).
ICC/IF Human
Chung H, Lee YS, Mayoral R et al. Omega-3 fatty acids reduce obesity-induced tumor progression independent of GPR120 in a mouse model of postmenopausal breast cancer. Oncogene. 2014 Sep 15 [PMID: 25220417] (WB, Mouse) WB Mouse
Show All 12 Publications.

Review for FFAR4/GPR120 Antibody (NBP1-00858) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-00858:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot FFAR4/GPR120 NBP1-00858
Enlarge
reviewed by:
Anonymous
WB Mouse 04/02/2015
View

Summary

ApplicationWestern Blot
Sample Testedwhole cell lysates from mouse bone marrow, spleen, bone marrow derived DCs/macrophages.
SpeciesMouse
LotNBP1-00858

Blocking

Blocking Details5% milk

Primary Anitbody

Dilution Ratio1:1000 in 1XTBST, 4 degree overnight

Secondary Antibody

Secondary Descriptiongoat anti rabbit IgG HRP conjugated
Secondary Concentration1:5000

Details

Detection Notesusing exposure machine

Comments

Commentsalthough the datasheet says it is for human, it looks like this Ab can detect mouse GPR120 as well

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FFAR4/GPR120 Antibody (NBP1-00858) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 488 Labeled NBP1-00858AF488
Alexa Fluor 647 Labeled NBP1-00858AF647
Biotin Labeled NBP1-00858B
DyLight 350 Labeled NBP1-00858UV
DyLight 405 Labeled NBP1-00858V
DyLight 488 Labeled NBP1-00858G
DyLight 550 Labeled NBP1-00858R
DyLight 650 Labeled NBP1-00858C
DyLight 680 Labeled NBP1-00858FR
DyLight 755 Labeled NBP1-00858IR
HRP Labeled NBP1-00858H

Additional FFAR4/GPR120 Products

Bioinformatics Tool for FFAR4/GPR120 Antibody (NBP1-00858)

Discover related pathways, diseases and genes to FFAR4/GPR120 Antibody (NBP1-00858). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Blogs on FFAR4/GPR120

There are no specific blogs for FFAR4/GPR120, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
04/02/2015
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol FFAR4
Entrez
Uniprot