FFAR4/GPR120 Antibody - BSA Free

Images

 
Genetic Strategies: Knockout Validated: FFAR4/GPR120 Antibody [NBP1-00858] - 3T3-L1 cells were exposed to DHA (100 i1/4M) for 2 d in the presence of the differentiation medium. The expression of GPR120, corin and ...read more
Immunohistochemistry-Paraffin: FFAR4/GPR120 Antibody [NBP1-00858] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse intestine using FFAR4/GPR120 antibody at 1:200 dilution. The intestinal ...read more
Western Blot: FFAR4/GPR120 Antibody [NBP1-00858] - Analysis of GPR120 (V259) antibody in extracts from LOVO cells.
Western Blot: FFAR4/GPR120 Antibody [NBP1-00858] - GPR120 expression in mouse spleen cells (1), bone marrow (2), bone marrow derived DC (3) and macrophages (4). This image was submitted through a verified customer ...read more
Immunocytochemistry/ Immunofluorescence: FFAR4/GPR120 Antibody [NBP1-00858] - GPR120 antibody was tested at 1:50 in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Immunohistochemistry-Paraffin: FFAR4/GPR120 Antibody [NBP1-00858] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse intestine using FFAR4/GPR120 antibody at 1:200 dilution. The intestinal ...read more
The combination of RA and omega-3 PUFAs induces Galphaq-P38 activation through RARalpha and GPR40(A): Cells were treated with RA(20uM) + EPA(80uM) with or without GPR120-knockdown for 15 min. Cell extracts were prepared ...read more
The expression of corin and ANP was increased in DHA-induced adipocytes. 3T3-L1 cells were exposed to DHA (100 uM) for 2 d in the presence of the differentiation medium. (D,E) The expression of corin and ANP was ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

FFAR4/GPR120 Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to an internal portion of the human GPR120 protein (between residues 200-300) [UniProt Q5NUL3]
Localization
FFAR4/GPR120 is a transmembrane protein with extracellular-helical-cytoplasmic domains
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FFAR4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry reported in scientific literature (PMID 34911928)
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Knockout Validated
  • Western Blot 1:500-1:1000
Reviewed Applications
Read 1 Review rated 5
using
NBP1-00858 in the following applications:

Publications
Read Publications using
NBP1-00858 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FFAR4/GPR120 Antibody - BSA Free

  • FFAR4
  • G protein-coupled receptor 120
  • G protein-coupled receptor 129
  • G protein-coupled receptor PGR4
  • GPR120
  • GPR129
  • G-protein coupled receptor 120
  • G-protein coupled receptor 129
  • G-protein coupled receptor GT01
  • G-protein coupled receptor PGR4
  • G-protein-coupled receptor GT01
  • GT01
  • MGC119984
  • O3FAR1
  • omega-3 fatty acid receptor 1
  • PGR4
  • PGR4DKFZp686F0824

Background

GPR120, a member of the rhodopsin family of G protein-coupled receptors (GPCRs), is a 377 amino acid protein which is expressed in the intestine. GPR120 is a receptor for unsaturated long-chain FFAs (free fatty acids). FFAs act as signaling molecules and are an important energy source. They also employ various physiological responses through their GPCRs. One such response occurs when dietary FFAs stimulate GPR120. This stimulation promotes the secretion of Glucagon-like peptide-1 (GLP-1) in vivo and in vitro.GLP-1 belongs to the class of molecules known as the incretins, which are associated with insulin secreted from the pancreas as a result of food intake. GLP-1 also inhibits Glucagon and gastric acid secretion and gastric emptying. Consequently, the role of GPR120 in the secretion of GLP-1 is critical in the treatment of diabetes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FFAR4/GPR120 Antibody (NBP1-00858)(21)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: FLOW, ICC/IF, IF/IHC, IHC-P, WB.


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FLOW
(1)
ICC/IF
(3)
IF/IHC
(2)
IHC-P
(1)
WB
(9)
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Human
(8)
Mouse
(8)
Rat
(4)
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Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using NBP1-00858 Applications Species
Karmokar PF, Moniri NH Free-Fatty Acid Receptor-4 (FFA4/GPR120) differentially regulates migration, invasion, proliferation and tumor growth of papillary renal cell carcinoma cells Biochemical pharmacology 2023-05-16 [PMID: 37201877]
Yu H, Yang W, Huang J et al. GPR120 induces regulatory dendritic cells by inhibiting HK2-dependent glycolysis to alleviate fulminant hepatic failure Cell death & disease 2021-12-16 [PMID: 34911928] (FLOW, Mouse) FLOW Mouse
Ito T, Yamamoto Y, Yamagishi N, Kanai Y Stomach secretes estrogen in response to the blood triglyceride levels Communications biology 2021-12-07 [PMID: 34876651] (IHC-P, Rat) IHC-P Rat
Kamakura R, Raza GS, Prasannan A et al. Dipeptidyl peptidase 4 and GLP-1 interplay in STC-1 and GLUTag cell lines Peptides 2020-09-27 [PMID: 32998057] (WB, Mouse) WB Mouse
Bae IS, Kim SH Expression and Secretion of an Atrial Natriuretic Peptide in Beige-Like 3T3-L1 Adipocytes Int J Mol Sci 2019-12-05 [PMID: 31817347] (WB, Human) WB Human
Bodis K, Kahl S, Simon MC et al. Reduced expression of stearoyl-CoA desaturase-1, but not free fatty acid receptor 2 or 4 in subcutaneous adipose tissue of patients with newly diagnosed type 2 diabetes mellitus. Nutr Diabetes 2018-09-07 [PMID: 30190473] (Human) Human
Liu D, Costanzo A, Evans MDM et al. Expression of the candidate fat taste receptors in human fungiform papillae and the association with fat taste function. Br. J. Nutr. 2018-07-01 [PMID: 29936924] (IF/IHC, Human) IF/IHC Human
Zhu S, Lin G, Song C et al. RA and O-3 PUFA co-treatment activates autophagy in cancer cells Oncotarget 2017-12-12 [PMID: 29312596] (WB, Human) WB Human
Kang S, Huang J, Lee BK et al. Omega-3 polyunsaturated fatty acids protect human hepatoma cells from developing steatosis through FFA4 (GPR120) Biochim. Biophys. Acta. 2017-11-07 [PMID: 29126901] (ICC/IF, Human) ICC/IF Human
Zhu S, Jiang X, Jiang S et al. GPR120 is not required for w-3 PUFAs-induced cell growth inhibition and apoptosis in breast cancer cells. Cell Biol. Int. 2017-10-05 [PMID: 28980737] (Human) Human
Show All 21 Publications.

Review for FFAR4/GPR120 Antibody (NBP1-00858) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-00858:
Filter by Applications
WB
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot FFAR4/GPR120 NBP1-00858
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5
reviewed by:
Verified Customer
WB Mouse 04/02/2015
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Summary

ApplicationWestern Blot
Sample Testedwhole cell lysates from mouse bone marrow, spleen, bone marrow derived DCs/macrophages.
SpeciesMouse
LotNBP1-00858

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FFAR4/GPR120 Antibody (NBP1-00858) (0)

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Secondary Antibodies

 

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Verified Customer
04/02/2015
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol FFAR4
Entrez
Uniprot