FFAR4/GPR120 Antibody - BSA Free

Knockout Validated: FFAR4/GPR120 Antibody [NBP1-00858] - 3T3-L1 cells were exposed to DHA (100 i1/4M) for 2 d in the presence of the differentiation medium. The expression of GPR120, corin and ANP was measured in the GPR120 deficient adipocytes treated with DHA by Western blotting. Image collected and cropped by Citeab from the following publication (Expression and Secretion of an Atrial Natriuretic Peptide in Beige-Like 3T3-L1 Adipocytes. Int J Mol Sci (2019) licensed under a CC-BY licence.

Immunohistochemistry-Paraffin: FFAR4/GPR120 Antibody [NBP1-00858] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse intestine using FFAR4/GPR120 antibody at 1:200 dilution. The intestinal epithelial cells showed an expected cytoplasmic staining (immunogen of NBP1-00858 corresponds to the cytoplasmic domain of GPR120 protein ), while some cells especially the goblet cells population depicted nuclear positivity also. The observed nuclear signal may be justified on the fact that - some GPCRs are known for nuclear translocation upon binding to extracellular or endogenous/non-secreted ligands, wherein they impact the trancriptional regulation via GPCR-heterotrimeric G-protein-effector complexes.

Western Blot: FFAR4/GPR120 Antibody [NBP1-00858] - Analysis of GPR120 (V259) antibody in extracts from LOVO cells.

Western Blot: FFAR4/GPR120 Antibody [NBP1-00858] - GPR120 expression in mouse spleen cells (1), bone marrow (2), bone marrow derived DC (3) and macrophages (4). This image was submitted through a verified customer review.

Immunocytochemistry/Immunofluorescence: FFAR4/GPR120 Antibody [NBP1-00858] - GPR120 antibody was tested at 1:50 in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.

Immunohistochemistry-Paraffin: FFAR4/GPR120 Antibody [NBP1-00858] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse intestine using FFAR4/GPR120 antibody at 1:200 dilution. The intestinal epithelial cells showed an expected cytoplasmic staining (immunogen of NBP1-00858 corresponds to the cytoplasmic domain of GPR120 protein), while some cells especially the goblet cells population depicted nuclear positivity also. The observed nuclear signal may be justified on the fact that - some GPCRs are known for nuclear translocation upon binding to extracellular or endogenous/non-secreted ligands, wherein they impact the trancriptional regulation via GPCR-heterotrimeric G-protein-effector complexes.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, IHC-P, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

FFAR4/GPR120 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to an internal portion of the human GPR120 protein (between residues 200-300) [UniProt Q5NUL3]
• Localization: FFAR4/GPR120 is a transmembrane protein with extracellular-helical-cytoplasmic domains
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FFAR4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Knockout Validated
  • Western Blot 1:500-1:1000
• Application Notes: Use in Flow Cytometry reported in scientific literature (PMID:34911928)..

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FFAR4/GPR120 Antibody - BSA Free

• FFAR4
• G protein-coupled receptor 120
• G protein-coupled receptor 129
• G protein-coupled receptor PGR4
• GPR120
• GPR129
• G-protein coupled receptor 120
• G-protein coupled receptor 129
• G-protein coupled receptor GT01
• G-protein coupled receptor PGR4
• G-protein-coupled receptor GT01
• GT01
• MGC119984
• O3FAR1
• omega-3 fatty acid receptor 1
• PGR4
• PGR4DKFZp686F0824

Background

GPR120, a member of the rhodopsin family of G protein-coupled receptors (GPCRs), is a 377 amino acid protein which is expressed in the intestine. GPR120 is a receptor for unsaturated long-chain FFAs (free fatty acids). FFAs act as signaling molecules and are an important energy source. They also employ various physiological responses through their GPCRs. One such response occurs when dietary FFAs stimulate GPR120. This stimulation promotes the secretion of Glucagon-like peptide-1 (GLP-1) in vivo and in vitro.GLP-1 belongs to the class of molecules known as the incretins, which are associated with insulin secreted from the pancreas as a result of food intake. GLP-1 also inhibits Glucagon and gastric acid secretion and gastric emptying. Consequently, the role of GPR120 in the secretion of GLP-1 is critical in the treatment of diabetes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FFAR4/GPR120 Antibody (NBP1-00858)(20)

Publications using NBP1-00858

• Yu H, Yang W, Huang J et al. GPR120 induces regulatory dendritic cells by inhibiting HK2-dependent glycolysis to alleviate fulminant hepatic failure Cell death & disease 2021-12-16 [PMID: 34911928] (FLOW, Mouse)
• Ito T, Yamamoto Y, Yamagishi N, Kanai Y Stomach secretes estrogen in response to the blood triglyceride levels Communications biology 2021-12-07 [PMID: 34876651] (IHC-P, Rat)
• Kamakura R, Raza GS, Prasannan A et al. Dipeptidyl peptidase 4 and GLP-1 interplay in STC-1 and GLUTag cell lines Peptides 2020-09-27 [PMID: 32998057] (WB, Mouse)
• Bae IS, Kim SH Expression and Secretion of an Atrial Natriuretic Peptide in Beige-Like 3T3-L1 Adipocytes Int J Mol Sci 2019-12-05 [PMID: 31817347] (WB, Human)
• Bodis K, Kahl S, Simon MC et al. Reduced expression of stearoyl-CoA desaturase-1, but not free fatty acid receptor 2 or 4 in subcutaneous adipose tissue of patients with newly diagnosed type 2 diabetes mellitus. Nutr Diabetes 2018-09-07 [PMID: 30190473] (Human)
• Liu D, Costanzo A, Evans MDM et al. Expression of the candidate fat taste receptors in human fungiform papillae and the association with fat taste function. Br. J. Nutr. 2018-07-01 [PMID: 29936924] (IHC, Human)
• Zhu S, Lin G, Song C et al. RA and O-3 PUFA co-treatment activates autophagy in cancer cells Oncotarget 2017-12-12 [PMID: 29312596] (WB, Human)
• Kang S, Huang J, Lee BK et al. Omega-3 polyunsaturated fatty acids protect human hepatoma cells from developing steatosis through FFA4 (GPR120) Biochim. Biophys. Acta. 2017-11-07 [PMID: 29126901] (ICC/IF, Human)
• Zhu S, Jiang X, Jiang S et al. GPR120 is not required for w-3 PUFAs-induced cell growth inhibition and apoptosis in breast cancer cells. Cell Biol. Int. 2017-10-05 [PMID: 28980737] (Human)
• Cheshmehkani A, Senatorov IS, Dhuguru J et al. Free-fatty acid receptor-4 (FFA4) modulates ROS generation and COX-2 expression via the C-terminal B-arrestin phosphosensor in Raw 264.7 macrophages Biochem. Pharmacol. 2017-09-21 [PMID: 28943238] (Mouse)
• Lee KP, Park SJ, Kang S et al. Omega-3 polyunsaturated fatty acids accelerate airway repair by activating FFA4 in club cells. Am. J. Physiol. Lung Cell Mol. Physiol. 2017-03-17 [PMID: 28314803] (WB, Mouse)
• Houthuijzen JM, Oosterom I, Hudson BD et al. Fatty acid 16:4(n-3) stimulates a GPR120-induced signaling cascade in splenic macrophages to promote chemotherapy resistance. FASEB J. 2017-02-09 [PMID: 28183801]
• Zhang D, So WY, Wang Y et al. Insulinotropic effects of GPR120 agonists are altered in obese non-diabetic and diabetic states. Clin. Sci. 2016-12-15 [PMID: 27980130] (Rat)
• Baron M, Veres A, Wolock SL et al. A Single-Cell Transcriptomic Map of the Human and Mouse Pancreas Reveals Inter- and Intra-cell Population Structure. Cell Syst 2016-10-26 [PMID: 27667365] (IHC, Mouse)
• Lee J, Moraes-Vieira Pm, Castoldi A et al. Branched fatty acid esters of hydroxy fatty acids (FAHFAs) protect against colitis by regulating the gut innate and adaptive immune systems. J. Biol. Chem. 2016-08-29 [PMID: 27573241] (WB)
• Anbazhagan AN, Priyamvada S, Gujral T et al. A Novel Anti-inflammatory Role of GPR120 in Intestinal Epithelial Cells. Am. J. Physiol., Cell Physiol. 2016-01-20 [PMID: 26791484] (ICC/IF, WB, Human, Mouse, Rat)
  
  Details:
  GPR120 antibody was used at 1:75 dilution in immunofluorescent / ICC-IF staining of control or agonist-treated (GW9508 or TUG-891, 6 hr) STC-1 cells grown on 6-well transwell inserts. The antibody was used for WB also on lysates from human, mouse or rat's intestinal mucosal tissues or from Caco-2 and STC-1 cell lines grown under several different experimental conditions.
• Kim JM, Lee KP, Park SJ et al. Omega-3 fatty acids induce Ca(2+) mobilization responses in human colon epithelial cell lines endogenously expressing FFA4. Acta Pharmacol. Sin. 2015-05-25 [PMID: 26005911] (ICC/IF, Human)
  
  Details:
  FFAR4/GPR120 antibody was used at 1:500 dilution in ICC-IF immunostaining assay for the detection of endogenous free fatty acid receptor 4 (FFA4) protein in HCT116 and HT-29 human colon cancer cells 30 min after exposing the cells to 50uM of alpha linolenic acid/LA, eicosapentaenoic acid / ELA or docosahexaenoic acid /DHA (Figure 1C and 1D).
• Chung H, Lee YS, Mayoral R et al. Omega-3 fatty acids reduce obesity-induced tumor progression independent of GPR120 in a mouse model of postmenopausal breast cancer. Oncogene. 2014-09-15 [PMID: 25220417] (WB, Mouse)
• Liu Y, Chen LY, Sokolowska M et al. The fish oil ingredient, docosahexaenoic acid, activates cytosolic phospholipase A2 via GPR120 receptor to produce prostaglandin E2 and plays an anti-inflammatory role in macrophages. Immunology 2014-03-28 [PMID: 24673159] (WB, Mouse)
• Jiang W, Zhu Z, McGinley JN et al. Identification of a molecular signature underlying inhibition of mammary carcinoma growth by dietary N-3 Fatty acids Cancer Res 2012-08-01 [PMID: 22651929] (WB, Rat)

Review for FFAR4/GPR120 Antibody (NBP1-00858) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: wei cao WB Mouse 04/02/2015

Summary

• Application: Western Blot
• Sample Tested: whole cell lysates from mouse bone marrow, spleen, bone marrow derived DCs/macrophages.
• Species: Mouse
• Lot: NBP1-00858

Bioinformatics

• Gene Symbol: FFAR4
• Entrez:
  • 338557(Human)
  • 294075(Rat)
• Uniprot:
  • Q5NUL3(Human)