FFAR4/GPR120 Antibody

Images

 
Genetic Strategies: Knockout Validated: FFAR4/GPR120 Antibody [NBP1-00858] - 3T3-L1 cells were exposed to DHA (100 i1/4M) for 2 d in the presence of the differentiation medium. The expression of GPR120, corin and ...read more
Immunohistochemistry-Paraffin: FFAR4/GPR120 Antibody [NBP1-00858] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse intestine using FFAR4/GPR120 antibody at 1:200 dilution. The intestinal ...read more
Western Blot: FFAR4/GPR120 Antibody [NBP1-00858] - Analysis of GPR120 (V259) antibody in extracts from LOVO cells.
Western Blot: FFAR4/GPR120 Antibody [NBP1-00858] - GPR120 expression in mouse spleen cells (1), bone marrow (2), bone marrow derived DC (3) and macrophages (4). This image was submitted through a verified customer ...read more
Immunocytochemistry/ Immunofluorescence: FFAR4/GPR120 Antibody [NBP1-00858] - GPR120 antibody was tested at 1:50 in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Immunohistochemistry-Paraffin: FFAR4/GPR120 Antibody [NBP1-00858] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse intestine using FFAR4/GPR120 antibody at 1:200 dilution. The intestinal ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
     

Genetic Strategies

   

FFAR4/GPR120 Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of the human GPR120 protein (between residues 200-300) [UniProt Q5NUL3]
Localization
FFAR4/GPR120 is a transmembrane protein with extracellular-helical-cytoplasmic domains
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FFAR4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Knockout Validated
Reviewed Applications
Read 1 Review rated 5
using
NBP1-00858 in the following applications:

Publications
Read Publications using
NBP1-00858 in the following applications:

  • 3 publications
  • IHC
    2 publications
  • WB
    9 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FFAR4/GPR120 Antibody

  • FFAR4
  • G protein-coupled receptor 120
  • G protein-coupled receptor 129
  • G protein-coupled receptor PGR4
  • GPR120
  • GPR129
  • G-protein coupled receptor 120
  • G-protein coupled receptor 129
  • G-protein coupled receptor GT01
  • G-protein coupled receptor PGR4
  • G-protein-coupled receptor GT01
  • GT01
  • MGC119984
  • O3FAR1
  • omega-3 fatty acid receptor 1
  • PGR4
  • PGR4DKFZp686F0824

Background

GPR120, a member of the rhodopsin family of G protein-coupled receptors (GPCRs), is a 377 amino acid protein which is expressed in the intestine. GPR120 is a receptor for unsaturated long-chain FFAs (free fatty acids). FFAs act as signaling molecules and are an important energy source. They also employ various physiological responses through their GPCRs. One such response occurs when dietary FFAs stimulate GPR120. This stimulation promotes the secretion of Glucagon-like peptide-1 (GLP-1) in vivo and in vitro.GLP-1 belongs to the class of molecules known as the incretins, which are associated with insulin secreted from the pancreas as a result of food intake. GLP-1 also inhibits Glucagon and gastric acid secretion and gastric emptying. Consequently, the role of GPR120 in the secretion of GLP-1 is critical in the treatment of diabetes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FFAR4/GPR120 Antibody (NBP1-00858)(18)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


Filter By Application
ICC/IF
(3)
IHC
(2)
WB
(9)
All Applications
Filter By Species
Human
(8)
Mouse
(7)
Rat
(3)
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using NBP1-00858 Applications Species
Kamakura R, Raza GS, Prasannan A et al. Dipeptidyl peptidase 4 and GLP-1 interplay in STC-1 and GLUTag cell lines Peptides Sep 27 2020 [PMID: 32998057] (WB, Mouse) WB Mouse
Bae IS, Kim SH Expression and Secretion of an Atrial Natriuretic Peptide in Beige-Like 3T3-L1 Adipocytes Int J Mol Sci Dec 5 2019 [PMID: 31817347] (WB, Human) WB Human
Bodis K, Kahl S, Simon MC et al. Reduced expression of stearoyl-CoA desaturase-1, but not free fatty acid receptor 2 or 4 in subcutaneous adipose tissue of patients with newly diagnosed type 2 diabetes mellitus. Nutr Diabetes Sep 7 2018 [PMID: 30190473] (Human) Human
Liu D, Costanzo A, Evans MDM et al. Expression of the candidate fat taste receptors in human fungiform papillae and the association with fat taste function. Br. J. Nutr. Jul 1 2018 [PMID: 29936924] (IHC, Human) IHC Human
Zhu S, Lin G, Song C et al. RA and O-3 PUFA co-treatment activates autophagy in cancer cells Oncotarget 2017 Dec 12 [PMID: 29312596] (WB, Human) WB Human
Kang S, Huang J, Lee BK et al. Omega-3 polyunsaturated fatty acids protect human hepatoma cells from developing steatosis through FFA4 (GPR120) Biochim. Biophys. Acta. 2017 Nov 07 [PMID: 29126901] (ICC/IF, Human) ICC/IF Human
Zhu S, Jiang X, Jiang S et al. GPR120 is not required for w-3 PUFAs-induced cell growth inhibition and apoptosis in breast cancer cells. Cell Biol. Int. 2017 Oct 05 [PMID: 28980737] (Human) Human
Cheshmehkani A, Senatorov IS, Dhuguru J et al. Free-fatty acid receptor-4 (FFA4) modulates ROS generation and COX-2 expression via the C-terminal B-arrestin phosphosensor in Raw 264.7 macrophages Biochem. Pharmacol. 2017 Sep 21 [PMID: 28943238] (Mouse) Mouse
Lee KP, Park SJ, Kang S et al. Omega-3 polyunsaturated fatty acids accelerate airway repair by activating FFA4 in club cells. Am. J. Physiol. Lung Cell Mol. Physiol. Mar 17 2017 [PMID: 28314803] (WB, Mouse) WB Mouse
Houthuijzen JM, Oosterom I, Hudson BD et al. Fatty acid 16:4(n-3) stimulates a GPR120-induced signaling cascade in splenic macrophages to promote chemotherapy resistance. FASEB J. 2017 Feb 09 [PMID: 28183801]
Show All 18 Publications.

Review for FFAR4/GPR120 Antibody (NBP1-00858) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-00858:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot FFAR4/GPR120 NBP1-00858
Enlarge
5
reviewed by:
Anonymous
WB Mouse 04/02/2015
View

Summary

ApplicationWestern Blot
Sample Testedwhole cell lysates from mouse bone marrow, spleen, bone marrow derived DCs/macrophages.
SpeciesMouse
LotNBP1-00858

Blocking

Blocking Details5% milk

Primary Anitbody

Dilution Ratio1:1000 in 1XTBST, 4 degree overnight

Secondary Antibody

Secondary Descriptiongoat anti rabbit IgG HRP conjugated
Secondary Concentration1:5000

Details

Detection Notesusing exposure machine

Comments

Commentsalthough the datasheet says it is for human, it looks like this Ab can detect mouse GPR120 as well

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FFAR4/GPR120 Antibody (NBP1-00858) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-00858AF350
Alexa Fluor 405 NBP1-00858AF405
Alexa Fluor 488 NBP1-00858AF488
Alexa Fluor 532 NBP1-00858AF532
Alexa Fluor 594 NBP1-00858AF594
Alexa Fluor 647 NBP1-00858AF647
Alexa Fluor 700 NBP1-00858AF700
Alexa Fluor 750 NBP1-00858AF750
Biotin NBP1-00858B
DyLight 350 NBP1-00858UV
DyLight 405 NBP1-00858V
DyLight 488 NBP1-00858G
DyLight 550 NBP1-00858R
DyLight 594 NBP1-00858DL594
DyLight 650 NBP1-00858C
DyLight 680 NBP1-00858FR
DyLight 755 NBP1-00858IR
FITC NBP1-00858F
HRP NBP1-00858H
Janelia Fluor 549 NBP1-00858JF549
Janelia Fluor 646 NBP1-00858JF646

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
04/02/2015
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol FFAR4
Entrez
Uniprot