GPIHBP1 Antibody

Images

 
Western Blot: GPIHBP1 Antibody [NB110-41537] - (1) Detection of GPIHBP1in transfected lysate and (2) empty vector lysate was used as a negative control.
Immunocytochemistry/ Immunofluorescence: GPIHBP1 Antibody [NB110-41537] - GPIHBP1 antibody was tested in A431 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Immunohistochemistry-Paraffin: GPIHBP1 Antibody [NB110-41537] - Analysis of FFPE mouse skeletal muscle using GPIHBP1 antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ChIP, Flow-CS, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.6 mg/ml

GPIHBP1 Antibody Summary

Immunogen
A synthetic peptide corresponding to an internal region [within residues 100-170] of mouse GPIHBP1. [Swiss-Prot# Q9D1N2]
Localization
Plasma membrane.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GPIHBP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:25 - 1:100
  • Immunohistochemistry-Paraffin 1:25 - 1:100
  • Flow (Cell Surface)
Application Notes
Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID: 24735886). Use in chromatin immunoprecipitation reported in scientific literature (PMID: 28099936). Use in FLOW cell surface reported in scientific literature (PMID: 24735886).
Control
Mouse Kidney Whole Tissue Lysate (Adult Whole Normal)
Control Peptide
GPIHBP1 Peptide (NB110-41537PEP)
Publications
Read Publications using
NB110-41537 in the following applications:

Reactivity Notes

Rat and Bovine reactivity reported in scientific literature (PMID: 24735886). Human reactivity reported in scientific literature (PMID: 28099936).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
0.6 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GPIHBP1 Antibody

  • glycosylphosphatidylinositol anchored high density lipoprotein binding protein1
  • glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein1
  • GPI anchored high density lipoprotein binding protein 1
  • GPI-Anchored HDL-Binding Protein 1
  • GPIHBP1
  • GPI-HBP1
  • GPI-HBP1LOC338328
  • HBP1
  • High density lipoprotein-binding protein 1
  • HYPL1D

Background

GPIHBP1 (glycosylphosphatidylinositol-anchored HDL-binding protein 1) is a glycosylphosphatidylinositol-anchored glycoprotein of capillary endothelial cells that shuttle lipoprotein lipase (LPL) from the interstitial spaces to capillary lumen, and is essential for triglyceride-rich lipoproteins metabolism in mammalian plasma. GPIHBP1 is localized on luminal/abluminal capillary endothelial cell surfaces where it is bound by a glycosylphosphatidylinositol anchor and associates strongly with LPL. It serves as LPL transporter from sub-endothelial spaces to luminal face of capillaries, enabling lipolysis of circulating triglycerides localized within plasma chylomicrons. It has high affinity for HDL and binds to LPL, chylomicrons as well as APOA5. In the absence of GPIHBP1, the stores of catalytically active LPL within tissues are normal, but the LPL is mislocalized to interstitial spaces and is absent from capillary lumen. LPL mislocalization interferes with lipoprotein lipolysis and causes chylomicronemia. In humans, loss of function GPIHBP1 mutations leads to familial chylomicronemia. It binds LPL and apoA-V strongly for serving as a platform for lipolysis within capillaries, particularly in tissues which show high expression levels for both GPIHBP1 and LPL genes, such as heart, skeletal muscle and adipose tissue. Gpihbp1-/Gpihbp1- knock out mice have shown that GPIHBP1-deficiency causes severe hypertriglyceridemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Bv
Applications: WB, ChIP, Flow-CS, ICC/IF, IHC, IHC-P

Publications for GPIHBP1 Antibody (NB110-41537)(7)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Bovine.

We have publications tested in 4 applications: ChIP, ICC/IF, IHC-P, WB.


Filter By Application
ChIP
(1)
ICC/IF
(4)
IHC-P
(1)
WB
(2)
All Applications
Filter By Species
Human
(2)
Mouse
(2)
Rat
(1)
Bovine
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB110-41537 Applications Species
Kim GT, Hahn KW, Sohn KY et al. PLAG enhances macrophage mobility for efferocytosis of apoptotic neutrophils via membrane redistribution of P2Y2 FEBS J. Nov 12 2019 [PMID: 31714686] (ICC/IF, Human) ICC/IF Human
Chan CY, Huang SY, Sheu JJ et al. Transcription factor HBP1 is a direct anti-cancer target of transcription factor FOXO1 in invasive oral cancer Oncotarget Jan 14 2017 [PMID: 28099936] (ChIP, Human) ChIP Human
Chiu AP, Wang F, Lal N et al. Endothelial cells respond to hyperglycemia by increasing the LPL transporter GPIHBP1. Am. J. Physiol. Endocrinol. Metab. 2014 Apr 15 [PMID: 24735886] (WB, IHC-P, ICC/IF, Rat, Bovine) WB, IHC-P, ICC/IF Rat, Bovine
Beigneux AP, Gin P, Davies BSJ et al. Highly Conserved Cysteines within the Ly6 Domain of GPIHBP1 Are Crucial for the Binding of Lipoprotein Lipase. J Biol Chem;284(44):30240-30247. 2009 [PMID: 19726683]
Davies BSJ, Waki H, Beigneux AP et al. The expression of GPIHBP1, an endothelial cell binding site for lipoprotein lipase and chylomicrons, is induced by peroxisome proliferator-activated receptor-gamma. Mol Endocrinol;22(11):2496-504. 2008 Nov. [PMID: 18787041] (ICC/IF, Mouse) ICC/IF Mouse
Gin P, Yin L, Davies BSJ et al. The acidic domain of GPIHBP1 is important for the binding of lipoprotein lipase and chylomicrons. J Biol Chem;283(43):29554-62. 2008 Oct 24. [PMID: 18713736]
Beigneux AP, Gin P, Davies BSJ et al. Glycosylation of Asn-76 in mouse GPIHBP1 is critical for its appearance on the cell surface and the binding of chylomicrons and lipoprotein lipase. J Lipid Res. 49(6):1312-21. 2008 Jun. [PMID: 18340083] (WB, ICC/IF, Mouse) WB, ICC/IF Mouse

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Product General Protocols

View specific protocols for GPIHBP1 Antibody (NB110-41537): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for GPIHBP1 Antibody (NB110-41537) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-41537AF350
Alexa Fluor 405 NB110-41537AF405
Alexa Fluor 488 NB110-41537AF488
Alexa Fluor 532 NB110-41537AF532
Alexa Fluor 594 NB110-41537AF594
Alexa Fluor 647 NB110-41537AF647
Alexa Fluor 700 NB110-41537AF700
Alexa Fluor 750 NB110-41537AF750
Biotin NB110-41537B
DyLight 350 NB110-41537UV
DyLight 405 NB110-41537V
DyLight 488 NB110-41537G
DyLight 550 NB110-41537R
DyLight 594 NB110-41537DL594
DyLight 650 NB110-41537C
DyLight 680 NB110-41537FR
DyLight 755 NB110-41537IR
FITC NB110-41537F
HRP NB110-41537H
Janelia Fluor 549 NB110-41537JF549
Janelia Fluor 646 NB110-41537JF646

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Bioinformatics

Gene Symbol GPIHBP1