GPIHBP1 Antibody

Western Blot: GPIHBP1 Antibody [NB110-41537] - (1) Detection of GPIHBP1in transfected lysate and (2) empty vector lysate was used as a negative control.

Immunocytochemistry/Immunofluorescence: GPIHBP1 Antibody [NB110-41537] - GPIHBP1 antibody was tested in A431 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Immunohistochemistry-Paraffin: GPIHBP1 Antibody [NB110-41537] - Analysis of FFPE mouse skeletal muscle using GPIHBP1 antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was observed on the luminal surface of capillary endothelium . Staining was performed by Histowiz.

Product Discontinued

GPIHBP1 Antibody Summary

• Immunogen: A synthetic peptide corresponding to an internal region [within residues 100-170] of mouse GPIHBP1. [Swiss-Prot# Q9D1N2]
• Localization: Plasma membrane.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GPIHBP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation
  • Flow (Cell Surface)
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:25 - 1:100
  • Immunohistochemistry-Paraffin 1:25 - 1:100
  • Western Blot 2 ug/ml
• Application Notes: Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID: 24735886). Use in chromatin immunoprecipitation reported in scientific literature (PMID: 28099936). Use in FLOW cell surface reported in scientific literature (PMID: 24735886).
• Control:

  Mouse Kidney Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Rat and Bovine reactivity reported in scientific literature (PMID: 24735886). Human reactivity reported in scientific literature (PMID: 28099936).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 0.6 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GPIHBP1 Antibody

• glycosylphosphatidylinositol anchored high density lipoprotein binding protein1
• glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein1
• GPI anchored high density lipoprotein binding protein 1
• GPI-Anchored HDL-Binding Protein 1
• GPIHBP1
• GPI-HBP1
• GPI-HBP1LOC338328
• HBP1
• High density lipoprotein-binding protein 1
• HYPL1D

Background

GPIHBP1 (glycosylphosphatidylinositol-anchored HDL-binding protein 1) is a glycosylphosphatidylinositol-anchored glycoprotein of capillary endothelial cells that shuttle lipoprotein lipase (LPL) from the interstitial spaces to capillary lumen, and is essential for triglyceride-rich lipoproteins metabolism in mammalian plasma. GPIHBP1 is localized on luminal/abluminal capillary endothelial cell surfaces where it is bound by a glycosylphosphatidylinositol anchor and associates strongly with LPL. It serves as LPL transporter from sub-endothelial spaces to luminal face of capillaries, enabling lipolysis of circulating triglycerides localized within plasma chylomicrons. It has high affinity for HDL and binds to LPL, chylomicrons as well as APOA5. In the absence of GPIHBP1, the stores of catalytically active LPL within tissues are normal, but the LPL is mislocalized to interstitial spaces and is absent from capillary lumen. LPL mislocalization interferes with lipoprotein lipolysis and causes chylomicronemia. In humans, loss of function GPIHBP1 mutations leads to familial chylomicronemia. It binds LPL and apoA-V strongly for serving as a platform for lipolysis within capillaries, particularly in tissues which show high expression levels for both GPIHBP1 and LPL genes, such as heart, skeletal muscle and adipose tissue. Gpihbp1-/Gpihbp1- knock out mice have shown that GPIHBP1-deficiency causes severe hypertriglyceridemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GPIHBP1 Antibody (NB110-41537)(7)

Publications using NB110-41537

• Kim GT, Hahn KW, Sohn KY et al. PLAG enhances macrophage mobility for efferocytosis of apoptotic neutrophils via membrane redistribution of P2Y2 FEBS J. 2019-11-12 [PMID: 31714686] (ICC/IF, Human)
• Chan CY, Huang SY, Sheu JJ et al. Transcription factor HBP1 is a direct anti-cancer target of transcription factor FOXO1 in invasive oral cancer Oncotarget 2017-01-14 [PMID: 28099936] (Chemotaxis, Human)
• Chiu AP, Wang F, Lal N et al. Endothelial cells respond to hyperglycemia by increasing the LPL transporter GPIHBP1. Am. J. Physiol. Endocrinol. Metab. 2014-04-15 [PMID: 24735886] (IHC-P, ICC/IF, WB, Rat, Bovine)
• Beigneux AP, Gin P, Davies BSJ et al. Highly Conserved Cysteines within the Ly6 Domain of GPIHBP1 Are Crucial for the Binding of Lipoprotein Lipase. J Biol Chem;284(44):30240-30247. 2009-01-01 [PMID: 19726683]
• Davies BSJ, Waki H, Beigneux AP et al. The expression of GPIHBP1, an endothelial cell binding site for lipoprotein lipase and chylomicrons, is induced by peroxisome proliferator-activated receptor-gamma. Mol Endocrinol;22(11):2496-504. 2008-11-01 [PMID: 18787041] (ICC/IF, Mouse)
• Gin P, Yin L, Davies BSJ et al. The acidic domain of GPIHBP1 is important for the binding of lipoprotein lipase and chylomicrons. J Biol Chem;283(43):29554-62. 2008-10-24 [PMID: 18713736]
• Beigneux AP, Gin P, Davies BSJ et al. Glycosylation of Asn-76 in mouse GPIHBP1 is critical for its appearance on the cell surface and the binding of chylomicrons and lipoprotein lipase. J Lipid Res. 49(6):1312-21. 2008-06-01 [PMID: 18340083] (WB, ICC/IF, Mouse)

Bioinformatics

• Gene Symbol: GPIHBP1