GPER/GPR30 Antibody - BSA Free

Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS4272] - Lung, non small cell carcinoma

Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS4272] - Analysis of anti-GPER1 / GPR30 antibody with human breast carcinoma at 10ug/ml.

Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS4272] - Analysis of anti-GPER1 / GPR30 antibody with human pancreas, carcinoma.

Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS4272] - Human Prostate, Carcinoma paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS4272] - Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE).

Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS4272] - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE).

Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS4272] - Prostate, Adenocarcinoma

Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS4272] - Breast, adenocarcinoma

• Reactivity: Hu, Rt, Ca, Pm, Pm
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

GPER/GPR30 Antibody - BSA Free Summary

• Description: Product can be stored undiluted at 4C for up to 1 month.
• Immunogen: Synthetic 18 amino acid peptide from C-terminus of human GPER/GPR30.
• Epitope: C-Terminus
• Localization: Endoplasmic reticulum - multi-pass membrane protein.
• Specificity: Human GPR30. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Predicted Species: Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GPER1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 5-10 ug/ml
  • Western Blot 1:100 - 1:2000
• Application Notes: Use in ICC/IF reported in scientific literature (PMID: 23822769). Use in IHC reported in scientific literature (PMID: 22378360)..

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Marmoset (100%), Mouse (89%), Hamster (89%), Bovine (83%).

Packaging, Storage & Formulations

• Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GPER/GPR30 Antibody - BSA Free

• CEPR
• CEPRG-protein coupled receptor 30
• Chemoattractant receptor-like 2
• chemokine receptor-like 2
• CMKRL2
• CMKRL2MGC99678
• constitutively expressed peptide-like receptor
• DRY12
• DRY12IL8-related receptor DRY12
• FEG-1
• FEG-1mER
• Flow-induced endothelial G-protein coupled receptor 1
• G protein-coupled estrogen receptor 1
• G protein-coupled receptor 30
• GPCR-BR
• GPER
• GPR30
• GPR30GPCR-Br
• G-protein coupled estrogen receptor 1
• heptahelix receptor
• LERGU
• LERGU2
• leucine rich protein in GPR30 3'UTR
• LyGPR
• Lymphocyte-derived G-protein coupled receptor
• Membrane estrogen receptor

Background

GPR30 has been reported in brain, breast carcinoma, blood, bone marrow, CNS, heart, liver, lung, lymph node, placenta, and spleen. ESTs have been isolated from B-cell/lung/testis, brain, ganglion, heart/melanocyte/uterus, kidney, tonsil, liver/spleen, placenta, and prostate libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GPER/GPR30 Antibody (NLS4272)(2)

Publications using NLS4272

• Srivastava DP, Evans PD et al. GPER 1: trials and tribulations of a Membrane Oestrogen Receptor. J Neuroendocrinol 2013-07-03 [PMID: 23822769] (ICC/IF, Rat)
• Spary EJ, Chapman SE, Sinfield JK, Maqbool A, Kaye J, Batten TF. Novel G Protein-Coupled Oestrogen Receptor GPR30 Shows Changes in mRNA Expression in the Rat Brain over the Oestrous Cycle. Neurosignals. 2012-02-23 [PMID: 22378360] (ICC/IF, IF/IHC, Rat)

Bioinformatics

• Gene Symbol: GPER1
• Entrez:
  • 2852(Human)
  • 76854(Mouse)
• Uniprot:
  • Q99527()