GPER/GPR30 Antibody

Images

 
Genetic Strategies: Western Blot: GPER/GPR30 Antibody [NBP1-31239] - A. 50 ug 293T whole cell lysate/extract. B. 50 ug whole cell lysate/extract of GFP-human GPR30-transfected 293T cells (No boiling). C. 50 ug ...read more
Immunocytochemistry/ Immunofluorescence: GPER/GPR30 Antibody [NBP1-31239] - Detection of GPER/GPR30 in HUVEC nucleus. Image courtesy of a product review by Dr. Subhadeep Chakrabarti of University of Alberta.
Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NBP1-31239] - Paraffin-embedded SNU1 xenograft, using antibody at 1:100 dilution.

Product Details

Summary
Reactivity Hu, Po, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

GPER/GPR30 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GPR30. The exact sequence is proprietary.
Localization
Cell membrane, Multi-pass membrane protein, Endoplasmic reticulum membrane, Multi-pass membrane protein, Golgi apparatus membrane, Multi-pass membrane protein
Predicted Species
Rat (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GPER1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
Application Notes
GPER/GPR30 antibody validated for ICC/IF from a verified customer review. Use in FLOW cytometry reported in scientific literature (PMID 27899250). IHC-P Assay dependent.
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-31239 in the following applications:

Publications
Read Publications using
NBP1-31239 in the following applications:

  • 1 publication
  • 1 publication
  • IHC
    1 publication
  • WB
    5 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for GPER/GPR30 Antibody

  • CEPR
  • CEPRG-protein coupled receptor 30
  • Chemoattractant receptor-like 2
  • chemokine receptor-like 2
  • CMKRL2
  • CMKRL2MGC99678
  • constitutively expressed peptide-like receptor
  • DRY12
  • DRY12IL8-related receptor DRY12
  • FEG-1
  • FEG-1mER
  • Flow-induced endothelial G-protein coupled receptor 1
  • G protein-coupled estrogen receptor 1
  • G protein-coupled receptor 30
  • GPCR-BR
  • GPER
  • GPR30
  • GPR30GPCR-Br
  • G-protein coupled estrogen receptor 1
  • heptahelix receptor
  • LERGU
  • LERGU2
  • leucine rich protein in GPR30 3'UTR
  • LyGPR
  • Lymphocyte-derived G-protein coupled receptor
  • Membrane estrogen receptor

Background

GPR30 a former orphan receptor has been linked to estrogen-mediated activation of adenylyl cyclase, release of epidermal growth factor (EGF)-related ligands, and specific estrogen binding.1 GPR30 acts independently of estrogen receptors, ERalpha and ERbeta, to release of heparin-binding EGF and may play a role in breast cancer tumorogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for GPER/GPR30 Antibody (NBP1-31239)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: FLOW, ICC/IF, IHC, WB.


Filter By Application
FLOW
(1)
ICC/IF
(1)
IHC
(1)
WB
(5)
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Filter By Species
Human
(6)
All Species

Review for GPER/GPR30 Antibody (NBP1-31239) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-31239:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence GPER/GPR30 NBP1-31239
Enlarge
4
reviewed by:
Anonymous
IF Human 03/05/2013
View

Summary

ApplicationImmunofluorescence
Sample TestedHuman umbilical vein endothelial cells
SpeciesHuman
Lot39224

Blocking

Blocking Details1% BSA in PBS for 60 min at RT

Primary Anitbody

Dilution Ratio1:150, overnight at 4C

Secondary Antibody

Secondary DescriptionGoat anti-Rabbit (red)
Secondary Concentration1:500

Details

Fixation DetailsFixed in 4% formalin for 20 min, permeabilized in 0.1% Triton-X-100 in PBS for 5min- both at RT

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GPER/GPR30 Antibody (NBP1-31239) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for GPER/GPR30 Antibody (NBP1-31239)

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anonymous
03/05/2013
Application: IF
Species: Human

Bioinformatics

Gene Symbol GPER1
Entrez
OMIM
Uniprot