GP-IX Antibody (A5E6-R)

Western Blot: GP-IX Antibody (A5E6-R) [NBP3-32152] - Western blot analysis of GP-IX on different lysates with Mouse anti-GP-IX antibody (NBP3-32152) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: Human lung tissue lysate Lane 3: Mouse spleen tissue lysate Lane 4: Mouse lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 19 kDa Observed band size: 28 kDa Exposure time: 1 minute 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32152) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: GP-IX Antibody (A5E6-R) [NBP3-32152] - Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Mouse anti-GP-IX antibody (NBP3-32152) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32152) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: A5E6-R
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

GP-IX Antibody (A5E6-R) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within N-terminal human GP-IX. (Uniprot: P14770)
• Localization: Membrane.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GP9
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:2000
  • Western Blot 1:2000
• Theoretical MW: 19 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for GP-IX Antibody (A5E6-R)

• CD42a antigen
• CD42a
• Glycoprotein 9
• glycoprotein IX (platelet)
• GP9
• GPIX
• GP-IX
• GPIXplatelet glycoprotein IX

Background

CD42a is a small membrane glycoprotein found on the surface of human platelets. It forms a one to one noncovalent complex with glycoprotein Ib, a platelet surface membrane glycoprotein complex that functions as a receptor for von Willebrand factor. The main portion of the receptor is a heterodimer composed of 2 polypeptide chains, an alpha chain and a beta chain, that are linked by disulfide bonds. The complete receptor complex includes noncovalent association of the alpha and beta subunits with GP9 and platelet glycoprotein V. CD42a is present on thrombocytes and also on a so far unidentified subpopulation of T lymphocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GP9