GOT2 Antibody - BSA Free

Western Blot: GOT2 Antibody [NBP2-32241] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-GOT2 antibody NBP2-32241 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry-Paraffin: GOT2 Antibody [NBP2-32241] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- GOT2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).

Immunohistochemistry-Paraffin: GOT2 Antibody [NBP2-32241] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- GOT2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).

• Reactivity: Hu, Mu, Po, Bv, Ch, Eq, Pm-Or, Rb, Xp
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

GOT2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit GOT2 Antibody - BSA Free (NBP2-32241) is a polyclonal antibody validated for use in IHC and WB. Anti-GOT2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen this antibody was made to, maps to a region between residue 380 to 430 of human Glutamic-Oxaloacetic Transaminase 2 using the numbering given in entry NP_002071.2 (GeneID 2806).
• Predicted Species: Porcine (100%), Orangutan (100%), Bovine (100%), Rabbit (100%), Chicken (100%), Equine (100%), Xenopus (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GOT2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation Negative
  • Western Blot 1:2000 - 1:10000
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GOT2 Antibody - BSA Free

• aspartate aminotransferase, mitochondrial
• EC 2.6.1
• EC 2.6.1.1
• FABP-1
• FABPpm
• Fatty acid-binding protein
• FLJ40994
• Glutamate oxaloacetate transaminase 2
• glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase2)
• KAT4
• KATIV
• kynurenine aminotransferase IV
• mAspAT
• mitAAT
• Plasma membrane-associated fatty acid-binding protein
• Transaminase A

Background

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GOT2 Antibody (NBP2-32241)(3)

Publications using NBP2-32241

• Rimmer L Investigating the metabolic changes that accompany skeletal muscle hypertrophy Thesis 2023-01-01 (Western Blot)
• Rimmer L, Geisbrecht E, Chao M et al. Investigating the metabolic changes that accompany skeletal muscle maturation Physiology 2023-05-01
• Park SJ, Yoo HC, Ahn E, Luo E et Al. Enhanced Glutaminolysis Drives Hypoxia-Induced Chemoresistance in Pancreatic Cancer Cancer Res 2023-01-03 [PMID: 36594876]

Bioinformatics

• Gene Symbol: GOT2
• Uniprot:
  • NP_002071.2(Human)