Goosecoid Antibody (3D11) - Azide and BSA Free

Western Blot: Goosecoid Antibody (3D11) [H00145258-M03] - Analysis of GSC expression in SJCRH30 (Cat # L027V1).

Western Blot: Goosecoid Antibody (3D11) [H00145258-M03] - Western blot analysis of GSC over-expressed 293 cell line, cotransfected with GSC Validated Chimera RNAi or non-transfected control. Blot probed with H00145258-M03. GAPDH (36.1 kDa) used as loading control.

Western Blot: Goosecoid Antibody (3D11) [H00145258-M03] - Analysis of GSC expression in COLO 320 HSR (Cat # L020V1).

Western Blot: Goosecoid Antibody (3D11) [H00145258-M03] - Analysis of GSC expression in transfected 293T cell line by GSC monoclonal antibody (M03), clone 3D11. Lane 1: GSC transfected lysatE (28.1 KDa). Lane 2: Non-transfected lysate.

ELISA: Goosecoid Antibody (3D11) [H00145258-M03] - Detection limit for recombinant GST tagged GSC is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, KD
• Clone: 3D11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Goosecoid Antibody (3D11) - Azide and BSA Free Summary

• Immunogen: GSC (NP_776248, 151 a.a. ~ 257 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LLNQLHCRRKRRHRTIFTDEQLEALENLFQETKYPDVGTREQLARKVHLREEKVEVWFKNRRAKWRRQKRSSSEESENAEKWNKTSSSKASPEKREEEGKSDLDSDS
• Specificity: GSC - goosecoid
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GSC
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for RNAi validation and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Goosecoid Antibody (3D11) - Azide and BSA Free

• goosecoid homeobox
• Goosecoid
• GSC
• homeobox protein goosecoid

Background

This gene encodes a member of the bicoid subfamily of the paired (PRD) homeobox family of proteins. The encoded protein acts as a transcription factor and may be autoregulatory. A similar protein in mice plays a role in craniofacial and rib cage development during embryogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GSC
• Entrez:
  • 145258(Human)
• OMIM:
  • 138890(Human)
• Uniprot:
  • NP_776248(Human)