GOLM1 Antibody (OTI4B12) - Azide and BSA Free

Western Blot: GOLM1 Antibody (OTI4B12) - Azide and BSA Free [NBP2-71916] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GOLM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GOLM1.

Immunocytochemistry/Immunofluorescence: GOLM1 Antibody (OTI4B12) - Azide and BSA Free [NBP2-71916] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GOLM1.

Immunohistochemistry: GOLM1 Antibody (OTI4B12) - Azide and BSA Free [NBP2-71916] - Staining of paraffin-embedded Human Kidney tissue using anti-GOLM1 mouse monoclonal antibody.

Flow Cytometry: GOLM1 Antibody (OTI4B12) - Azide and BSA Free [NBP2-71916] - Analysis of Hela cells, using anti-GOLM1 antibody , (Red), compared to a nonspecific negative control antibody, (Blue).

Western Blot: GOLM1 Antibody (OTI4B12) - Azide and BSA Free [NBP2-71916] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GOLM1 monoclonal antibody.

Immunohistochemistry: GOLM1 Antibody (OTI4B12) - Azide and BSA Free [NBP2-71916] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GOLM1 mouse monoclonal antibody.

Immunohistochemistry: GOLM1 Antibody (OTI4B12) - Azide and BSA Free [NBP2-71916] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-GOLM1 mouse monoclonal antibody.

Immunohistochemistry: GOLM1 Antibody (OTI4B12) - Azide and BSA Free [NBP2-71916] - Staining of paraffin-embedded Human colon tissue using anti-GOLM1 mouse monoclonal antibody.

Immunohistochemistry: GOLM1 Antibody (OTI4B12) - Azide and BSA Free [NBP2-71916] - Staining of paraffin-embedded Human endometrium tissue using anti-GOLM1 mouse monoclonal antibody.

Immunohistochemistry: GOLM1 Antibody (OTI4B12) - Azide and BSA Free [NBP2-71916] - Staining of paraffin-embedded Human prostate tissue using anti-GOLM1 mouse monoclonal antibody.

• Reactivity: Hu, Pm, Pm
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI4B12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

GOLM1 Antibody (OTI4B12) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human GOLM1(NP_808800) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GOLM1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:200-500
• Theoretical MW: 45.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for GOLM1 Antibody (OTI4B12) - Azide and BSA Free

• bA379P1.3
• FLJ23608
• golgi membrane protein 1
• Golgi membrane protein GP73
• Golgi phosphoprotein 2C9orf155
• golgi protein, 73-kD
• GOLPH2 FLJ22634
• GP73 chromosome 9 open reading frame 155
• GP73
• PSEC0257

Background

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by GOLM1 is a type II Golgi transmembrane protein. It processes protein synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of GOLM1 has been observed to be upregulated in response to viral infection. Two alternatively spliced transcript variants encoding the same protein have been described for GOLM1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GOLM1