Golgi Glycoprotein 1/GLG1 Antibody (GLG1/970)

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Immunohistochemistry-Paraffin: Golgi Glycoprotein 1/GLG1 Antibody (GLG1/970) [NBP2-44361] - Human Pancreas stained with GLG1 Monoclonal Antibody (GLG1/970).

Product Details

Summary
Reactivity Hu, Mu(-), Rt(-)Species Glossary
Applications WB, Flow, IHC-P, ICC/IF
Clone
GLG1/970
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Golgi Glycoprotein 1/GLG1 Antibody (GLG1/970) Summary

Immunogen
Golgi fraction from human liver cells
Localization
Golgi complex in cytoplasm
Marker
Marker for Human Cells
Specificity
This MAb recognizes a protein of 134kDa, which binds fibroblast growth factor and E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils). Fucosylation is essential for binding to E-selectin. It contains sialic acid residues and 16 Cys-rich GLG1 repeats. This MAb can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae. The Golgi tends to be more pronounced and numerous in cells that make and secrete many substances such as plasma B cells.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
GLG1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 1.0 ug/ml
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1ml
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
  • Immunocytochemistry 0.5 - 1.0 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
Application Notes
Hu-chromosome location: 16q22.3
Immunocytochemistry Acetone or paraformaldehyde fixed 0.5 - 1 ug/ml for 30 min; Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes.
Theoretical MW
134 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10mM PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Golgi Glycoprotein 1/GLG1 Antibody (GLG1/970)

  • CFR1
  • CFR-1
  • cysteine-rich fibroblast growth factor receptor
  • DKFZp686L08213
  • E-selectin ligand 1
  • ESL1
  • ESL-1
  • FLJ23319
  • FLJ23967
  • FLJ32797
  • GLG1
  • Golgi apparatus protein 1
  • Golgi Glycoprotein 1
  • golgi sialoglycoprotein MG-160
  • MG160
  • MG-160

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GLG1
Uniprot