GOLGA5 Antibody (GT2726) - Azide and BSA Free

Western Blot: GOLGA5 Antibody (GT2726) [NBP3-13566] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GOLGA5 antibody [GT2726] (NBP3-13566) diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: GOLGA5 Antibody (GT2726) [NBP3-13566] - GOLGA5 antibody [GT2726] detects GOLGA5 protein at Golgi apparatus by immunofluorescent analysis. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GOLGA5 protein stained by GOLGA5 antibody [GT2726] (NBP3-13566) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.

• Reactivity: Hu, Bv, RM
• Applications: WB, ICC/IF
• Clone: GT2726
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

GOLGA5 Antibody (GT2726) - Azide and BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human GOLGA5. The exact sequence is proprietary.
• Predicted Species: Rhesus Macaque (98%), Bovine (90%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GOLGA5
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 83 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse (89%), Rat (88%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for GOLGA5 Antibody (GT2726) - Azide and BSA Free

• Cell proliferation-inducing gene 31 protein
• golgi autoantigen, golgin subfamily a, 5
• golgin A5
• Golgin subfamily A member 5
• Golgin-84
• GOLIM5
• Protein Ret-II
• PTC5
• RET-fused gene 5 protein
• RETII
• ret-II
• rfg5
• RFG5golgi integral membrane protein 5

Background

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes a member of the golgin family of proteins, whose members localize to the Golgi. This protein is a coiled-coil membrane protein that has been postulated to play a role in vesicle tethering and docking. Translocations involving this gene and the ret proto-oncogene have been found in tumor tissues; the chimeric sequences have been designated RET-II and PTC5. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GOLGA5