GO Protein alpha Antibody

Western Blot: GO Protein alpha Antibody [NBP2-16703] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAO1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: GO Protein alpha Antibody [NBP2-16703] - DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GNAO1 stained by GNAO1 antibody diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [1338] (GTX631831) diluted at 1:500. Blue: Fluoroshield with DAPI.

Immunohistochemistry-Frozen: GO Protein alpha Antibody [NBP2-16703] - Frozen-sectioned mouse cerebellum. Green: GNAO1 stained by GNAO1 antibody diluted at 1:250. Red: NF-H, stained by NF-H antibody [114] diluted at 1:500. Blue: Fluoroshield with DAPI.

Western Blot: GO Protein alpha Antibody [NBP2-16703] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAO1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: GO Protein alpha Antibody [NBP2-16703] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAO1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: GO Protein alpha Antibody [NBP2-16703] - Colon carcinoma. GNAO1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: GO Protein alpha Antibody [NBP2-16703] - Mouse pancreas. GNAO1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: GO Protein alpha Antibody [NBP2-16703] - Rat pancreas. GNAO1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunoprecipitation: GO Protein alpha Antibody [NBP2-16703] - GNAO1 protein from HeLa whole cell extracts using 5 ug of GNAO1 antibody (NBP2-16703). Western blot analysis was performed using GNAO1 antibody (NBP2-16703). EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Immunohistochemistry-Paraffin: GO Protein alpha Antibody [NBP2-16703] - Analysis of paraffin-embedded zebrafish tissue, using GNAO1 antibody at 1:300 dilution.

• Reactivity: Hu, Mu, Rt, Ze
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

GO Protein alpha Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human GO Protein alpha. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GNAO1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:20000
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for GO Protein alpha Antibody

• DKFZp686O0962
• G-ALPHA-o
• GNAO
• guanine nucleotide binding protein (G protein), alpha activating activitypolypeptide O
• guanine nucleotide binding protein, alpha activating polypeptide O
• guanine nucleotide-binding protein G(o) subunit alpha

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for GO Protein alpha Antibody (NBP2-16703)(1)

Publication using NBP2-16703

• Chen S, Zhi Z et al. Bright Light Suppresses Form-Deprivation Myopia Development With Activation of Dopamine D1 Receptor Signaling in the ON Pathway in Retina. Invest Ophthalmol Vis Sci 2017-01-04 [PMID: 28431434] (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: GNAO1
• Uniprot:
  • P09471(Human)