CaMKII beta Antibody

Images

 
Western Blot: CaMKII beta Antibody [NB120-22131] - Analysis of CAMKIIb in rat brain lysate using NB120-22131 at 1:2000.
Immunocytochemistry/ Immunofluorescence: CaMKII beta Antibody [NB120-22131] - Staining of CAMKIIb using NB120-22131 in rat hippocampal neurons at a dilution of 1:100, note that staining is rather weak.
Western Blot: CaMKII beta Antibody [NB120-22131] - Analysis of CAMKII beta in A) human, B) mouse and C) rat brain lysate using CAMKII beta antibody at 1:2000.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit

Order Details

CaMKII beta Antibody Summary

Immunogen
This antibody was developed against a synthetic peptide corresponding to amino acids 499-513 (VRRGSGTPEAEAPRQ) of human CAMKII beta.
Localization
Cytoplasmic, widely expressed; expressed in adult and fetal brain. (Expression is lightly lower in fetal brain.)
Specificity
Human CAMKII beta GenBank no. GENE ID: 816 CAMK2B is a 664 amino acid protein. However, several isoforms of varying amino acid sizes have been described for CAMKII beta. Researchers are encouraged to utilize the sequence information used for immunogen (VRRGSGTPEAEAPRQ) to gain additional information about these isoforms and various molecular weights which may be detected by western blot analysis.
Clonality
Polyclonal
Host
Rabbit
Gene
CAMK2B
Purity
Unpurified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
Whole antisera with 50% Glycerol
Preservative
0.025% Sodium Azide
Purity
Unpurified

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence 1:100
Publications
Read Publications using
NB120-22131 in the following applications:

  • 1 publication
  • WB
    2 publications

Alternate Names for CaMKII beta Antibody

  • calcium/calmodulin-dependent protein kinase (CaM kinase) II beta
  • calcium/calmodulin-dependent protein kinase II beta
  • calcium/calmodulin-dependent protein kinase type II beta chain
  • calcium/calmodulin-dependent protein kinase type II subunit beta
  • CaM kinase II beta subunit
  • CaM kinase II subunit beta
  • CAM2CAMK2
  • CAMKB
  • CaMK-II subunit beta
  • CaM-kinase II beta chain
  • EC 2.7.11
  • EC 2.7.11.17
  • MGC29528
  • proline rich calmodulin-dependent protein kinase

Background

The multifunctional CaMKII beta, or Ca2+/calmodulin-dependent protein kinase II, is a well known effector of calcium- and calmodulin- mediated functions. It is present in many tissues but is most abundant in the brain. CaMKII is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. Autophosphorylation plays an important role in the regulation of the kinase activity. CaMKII is required for synaptic plasticity, as in Long Term Potentiation (LTP), a cellular model for learning and memory.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CaMKII beta Antibody (NB120-22131)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: IHC-P, WB.


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IHC-P
(1)
WB
(2)
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(2)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol CAMK2B
Entrez

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