GMEB1 Antibody

Western Blot: GMEB1 Antibody [NBP1-46207] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-GMEB1 antibody NBP1-46207used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. GMEB1 was also immunoprecipitated by another rabbit anti-GMEB1 antibody, which recognizes an upstream epitope.

Immunohistochemistry-Paraffin: GMEB1 Antibody [NBP1-46207] - Sample: FFPE section of human lung carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-GMEB1 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

GMEB1 Antibody Summary

• Immunogen: The immunogen for this product maps to a region between residue 523 and 573 of human Glucocorticoid Modulatory Element Binding Protein 1, using the numbering given in entry NP_006573.2 (GeneID 10691).
• Predicted Species: Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GMEB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GMEB1 Antibody

• DNA-binding protein p96PIF
• glucocorticoid modulatory element binding protein 1
• glucocorticoid modulatory element-binding protein 1
• GMEB-1
• P96PIF
• Parvovirus initiation factor p96
• PIF p96
• PIF96

Background

This gene is a member of KDWK gene family. The product of this gene associates with GMEB2 protein, and the complex is essential for parvovirus DNA replication. Study of rat homolog implicates the role of this gene in modulation of transactivation by the glucocorticoid receptor bound to glucocorticoid response elements. Two alternative spliced transcript variants encoding different isoforms exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GMEB1
• Entrez:
  • 10691(Human)
• Uniprot:
  • Q9Y692(Human)