Importin alpha 2/KPNA2 Antibody

Images

 
Western Blot: Importin alpha 2/KPNA2 Antibody [NB100-79807] - Whole cell lysate from HeLa (5, 15 and 50 ug) and 293T (50 ug) cells. Antibody used at 0.1 ug/ml.
Immunohistochemistry: Importin alpha 2/KPNA2 Antibody [NB100-79807] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RCH1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

Order Details

Importin alpha 2/KPNA2 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human RAG cohort 1 (Karyopherin Alpha 2) using the numbering given in entry NP_002257.1 (GeneID 3838).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KPNA2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:15000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-10 mg/mg
Application Notes
Use in Immunoprecipitation reported in scientific literature (PMID 25977366). Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Positive Control
Importin alpha 2/KPNA2 Lysate (NBL1-12370)
Publications
Read Publications using
NB100-79807 in the following applications:

  • IP
    1 publication
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Importin alpha 2/KPNA2 Antibody

  • Importin alpha 1
  • Importin alpha 2
  • importin-alpha-P1
  • IPOA1
  • karyopherin alpha 2 (RAG cohort 1, importin alpha 1)
  • Karyopherin subunit alpha-2
  • KPNA2
  • Pendulin
  • QIP2
  • QIP2importin alpha 2
  • RAG cohort 1
  • RAG cohort protein 1
  • RCH1
  • RCH1importin subunit alpha-2
  • SRP1
  • SRP1alpha
  • SRP1-alpha

Background

The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Importin alpha 2/KPNA2 Antibody (NB100-79807)(6)

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Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol KPNA2
Entrez
Uniprot