Home » GM130/GOLGA2 » GM130/GOLGA2 Antibodies » GM130/GOLGA2 Antibody

GM130/GOLGA2 Antibody

 Submit a Review

Images

 
(
Enlarge)
Western Blot: GM130/GOLGA2 Antibody [H00002801-B02P] - Analysis of GOLGA2 expression in human placenta.
Western Blot: GM130/GOLGA2 Antibody [H00002801-B02P] - Analysis of GOLGA2 expression in human placenta.
Immunocytochemistry/ Immunofluorescence: GM130/GOLGA2 Antibody [H00002801-B02P] - Analysis of purified antibody to GOLGA2 on HepG2 cell. (antibody concentration 7 ug/ml)
Western Blot: GM130/GOLGA2 Antibody [H00002801-B02P] - Analysis of GOLGA2 expression in transfected 293T cell line by GOLGA2 polyclonal antibody. Lane1:GOLGA2 transfected lysate(108.9 KDa). Lane2:Non-transfected lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

100% Guarantee on every product

GM130/GOLGA2 Antibody Summary

Immunogen
GOLGA2 (NP_004477.2, 1 a.a. - 990 a.a.) full-length human protein. MSEETRQSKLAAAKKKLREYQQRNSPGVPTGAKKKKKIKNGSNPETTTSGGCHSPEDTPKDNAATLQPSDDTVLPGGVPSPGASLTSMAASQNHDADNVPNLMDETKTFSSTESLRQLSQQLNGLVCESATCVNGEGPASSANLKDLESRYQQLAVALDSSYVTNKQLNITIEKLKQQNQEITDQLEEEKKECHQKQGALREQLQVHIQTIGILVSEKAELQTALAHTQHAARQKEGESEDLASRLQYSRRRVGELERALSAVSTQQKKADRYNKELTKERDALRLELYKNTQSNEDLKQEKSELEEKLRVLVTEKAGMQLNLEELQKKLEMTELLLQQFSSRCEAPDANQQLQQAMEERAQLEAHLGQVMESVRQLQMERDKYAENLKGESAMWRQRMQQMSEQVHTLREEKECSMSRVQELETSLAELRNQMAEPPPPEPPAGPSEVEQQLQAEAEHLRKELEGLAGQLQAQVQDNEGLSRLNREQEERLLELERAAELWGEQAEARRQILETMQNDRTTISRALSQNRELKEQLAELQSGFVKLTNENMEITSALQSEQHVKRELGKKLGELQEKLSELKETVELKSQEAQSLQQQRDQYLGHLQQYVAAYQQLTSEKEVLHNQLLLQTQLVDQLQQQEAQGKAVAEMARQELQETQERLEAATQQNQQLRAQLSLMAHPGEGDGLDREEEEDEEEEEEEAVAVPQPMPSIPEDLESREAMVAFFNSAVASAEEEQARLRGQLKEQRVRCRRLAHLLASAQKEPEAAAPAPGTGGDSVCGETHRALQGAMEKLQSRFMELMQEKADLKERVEELEHRCIQLSGETDTIGEYIALYQSQRAVLKERHREKEEYISRLAQDKEEMKVKLLELQELVLRLVGDRNEWHGRFLAAAQNPADEPTSGAPAPQELGAANQQGDLCEVSLAGSVEPAQGEAREGSPRDNPTAQQIMQLLREMQNPRERPGLGSNPCIPFFYRADENDEVKITVI
Marker
Golgi Apparatus Marker
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
GOLGA2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
Application Notes
It has been used for WB, Functional and IF.
Control
GM130/GOLGA2 Lysate (NBP2-65274)

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein G purified

Notes

Quality control test: Antibody reactive against mammalian transfected lysate.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for GM130/GOLGA2 Antibody

  • 130 kDa cis-Golgi matrix protein
  • GM130 autoantigen
  • GM130
  • GM130Golgi matrix protein GM130
  • GOLGA2
  • golgi autoantigen, golgin subfamily a, 2
  • golgin A2
  • Golgin subfamily A member 2
  • Golgin-95
  • MGC20672
  • SY11 Protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

GOLGB1/Giantin
Antibody
NBP2-22321
Western Blot: GOLGB1/Giantin Antibody [NBP2-22321] - HeLa cell lysate sample treated with DMSO was run on 8% SDS-PAGE. Both giantin monomer and dimer are indicated. Image from verified customer review.
Western Blot: GOLGB1/Giantin Antibody [NBP2-22321] - Whole cell lysate (50 ug) from 293T, HeLa, and Jurkat cells. NBP2-22321 used for WB at 0.1 ug/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunoprecipitation: GOLGB1/Giantin Antibody [NBP2-22321] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22321 used for IP at 6 ug/mg lysate. GOLGB1 was also immunoprecipitated by rabbit anti-GOLGB1 antibodies NBP2-22322 and NBP2-22323. For blotting immunoprecipitated GOLGB1, NBP2-22321 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
(1 Review)
(3 Publications)
Species: Hu
Applications: WB, ICC/IF, IP, IF
VDP p115 Antibody
NBP2-38401
Western Blot: VDP p115 Antibody [NBP2-38401] - Analysis using Anti-USO1 antibody NBP2-38401 (A) shows similar pattern to independent antibody NBP2-55590 (B).
Immunocytochemistry/Immunofluorescence: VDP p115 Antibody [NBP2-38401] - Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.
Immunohistochemistry: VDP p115 Antibody [NBP2-38401] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
ARHGAP4 Antibody
NBP1-88527
Western Blot: ARHGAP4 Antibody [NBP1-88527] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.
Immunohistochemistry-Paraffin: ARHGAP4 Antibody [NBP1-88527] - Staining of human colon.
Western Blot: ARHGAP4 Antibody [NBP1-88527] - Analysis in human cell line RPMI-8226.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
GRASP65 Antibody
(OTI5G8)
NBP2-02665
Western Blot: GRASP65 Antibody (5G8) [NBP2-02665] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GRASP65 monoclonal antibody.
Immunocytochemistry: GRASP65 Antibody (5G8) [NBP2-02665] - Golgi localization in human Wi26 cells. Works for ICC on MeOH and PFA-fixed cells 1:100-1:200 dilution WB on human lung fibroblasts 1:2000 in TBST (membrane blocking with 5% skimmed milk). This image was submitted via customer Review.
Immunohistochemistry-Paraffin: GRASP65 Antibody (5G8) [NBP2-02665] - Staining of paraffin-embedded Human prostate tissue using anti-GRASP65 mouse monoclonal antibody.
(3 Reviews)
Species: Hu, Rt, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P, ICC
GOLPH3 Antibody
NBP2-75515
Immunocytochemistry/Immunofluorescence: GOLPH3 Antibody [NBP2-75515] - Staining GOLPH3 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: GOLPH3 Antibody [NBP2-75515] - Analysis of paraffin-embedded mouse stomach tissue using anti-GOLPH3 antibody. Counter stained with hematoxylin.
Flow Cytometry: GOLPH3 Antibody [NBP2-75515] - Analysis of Hela cells with GOLPH3 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
TGN46 Antibody
NBP1-49643
Knockout Validated: TGN46 Antibody [NBP1-49643] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and TGN46 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human TGN46 Polyclonal Antibody (NBP1-49643) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). Specific band was detected for TGN46 at approximately 100 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Western Blot: TGN46 Antibody [NBP1-49643] - Analysis of extracts from HeLa cells using TGN46 antibody (NBP1-49643, 1:100).
Immunocytochemistry/Immunofluorescence: TGN46 Antibody [NBP1-49643] - T98G glioblastoma cells probed for Golgi (Alexa Fluor 488 conjugated TGN-46 antibody, Green), tubulin (Alexa Fluor 594, Red), and nucleus (DAPI, blue). Image from the Alexa Fluor 488 version of this antibody. Image from verified customer review.
(2 Reviews)
(8 Publications)
Species: Hu, Mk
Applications: WB, Flow, ICC/IF, IHC, IHC-P, Flow-IC, KO
Rab1A Antibody
NBP1-55113
Western Blot: Rab1A Antibody [NBP1-55113] - Reccomended Titration: 0.2 - 1 ug/ml ELISA Titer: 1:312500 Positive Control: Human Muscle
Immunohistochemistry: Rab1A Antibody [NBP1-55113] - Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue Observed Staining: Cytoplasmic in excellent staining Primary Antibody Concentration: 1:100 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec
Western Blot: Rab1A Antibody [NBP1-55113] - HeLa, Vera, HeLa transfected with mouse construct, Antibody Titration: 0.2-1 ug/ml
Species: Hu, Mu
Applications: WB, IHC, IHC-P
GOLGA3 Antibody
NBP1-91953
Western Blot: GOLGA3 Antibody [NBP1-91953] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunocytochemistry/Immunofluorescence: GOLGA3 Antibody [NBP1-91953] - Immunofluorescent staining of human cell line A-431 shows localization to nucleus, nucleoli & the Golgi apparatus.
Immunohistochemistry-Paraffin: GOLGA3 Antibody [NBP1-91953] - Staining of human kidney.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NSF Antibody
NBP1-87035
Western Blot: NSF Antibody [NBP1-87035] - Analysis in control (vector only transfected HEK293T lysate) and NSF over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/Immunofluorescence: NSF Antibody [NBP1-87035] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Immunohistochemistry-Paraffin: NSF Antibody [NBP1-87035] - Staining in human cerebral cortex and liver tissues using anti-NSF antibody. Corresponding NSF RNA-seq data are presented for the same tissues.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
SAR1 Antibody
NBP1-80789
Western Blot: SAR1 Antibody [NBP1-80789] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-SAR1A antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunohistochemistry-Paraffin: SAR1 Antibody [NBP1-80789] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
Western Blot: SAR1 Antibody [NBP1-80789] - Analysis in human cell line A-431.
Species: Hu
Applications: WB, IHC, IHC-P, KD
Golgin 97 Antibody
NBP2-38528
Western Blot: Golgin 97 Antibody [NBP2-38528] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue
Immunocytochemistry/Immunofluorescence: Golgin 97 Antibody [NBP2-38528] - Immunofluorescent staining of human cell line A549 shows localization to the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Golgin 97 Antibody [NBP2-38528] - Staining in human testis and pancreas tissues using anti-GOLGA1 antibody. Corresponding GOLGA1 RNA-seq data are presented for the same tissues.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
EEA1 Antibody
(6D4.1D4)
NBP2-36568
Western Blot: EEA1 Antibody (6D4.1D4) [NBP2-36568] - Analysis of partial recombinant human EEA1 protein (14kDa) with EEA1 antibody clone 6D4.1D4 at 0.5 ug mL.
Immunohistochemistry-Paraffin: EEA1 Antibody (6D4.1D4) [NBP2-36568] - Analysis of FFPE tissue section of human testes using mouse monoclonal EEA1 antibody (clone 6D4.1D4) at 10 ug/ml concentration. The interstitial cells depicted a punctate to granular cytoplasmic staining pattern for EEA1 protein.
Flow (Intracellular): EEA1 Antibody (6D4.1D4) [NBP2-36568] - Intracellular staining of human EEA1 in Flow cytometry using 2.5 ug of antibody per 1 million cells. Isotype control was mouse IgG1.
Species: Hu, Mu
Applications: WB, IHC, IHC-P, Flow-IC
IQGAP1 Antibody
(JG38-74)
NBP2-75545
Western Blot: IQGAP1 Antibody (JG38-74) [NBP2-75545] - Analysis of IQGAP1 on different lysates using anti-IQGAP1 antibody at 1/1,000 dilution.Positive control:Lane 1: HUVEC Lane 2: A431Lane 3: Mouse placenta
Immunocytochemistry/Immunofluorescence: IQGAP1 Antibody (JG38-74) [NBP2-75545] - Staining IQGAP1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: IQGAP1 Antibody (JG38-74) [NBP2-75545] - Analysis of paraffin-embedded mouse colon tissue using anti-IQGAP1 antibody. Counter stained with hematoxylin.
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
GRASP55 Antibody
NBP1-89747
Western Blot: GRASP55 Antibody [NBP1-89747] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GORASP2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/Immunofluorescence: GRASP55 Antibody [NBP1-89747] - Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.
Immunohistochemistry-Paraffin: GRASP55 Antibody [NBP1-89747] - Staining of human epididymis shows strong cytoplasmic positivity in glandular cells.
(2 Reviews)
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, KD
CDC2/CDK1 Antibody
NBP1-85729
Western Blot: CDC2/CDK1 Antibody [NBP1-85729] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/Immunofluorescence: CDC2/CDK1 Antibody [NBP1-85729] - Staining of human cell line U-251 MG shows localization to nucleus & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining in human tonsil and pancreas tissues. Corresponding CDK1 RNA-seq data are presented for the same tissues.
(3 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
GOLGA5 Antibody
NBP1-83352
Western Blot: GOLGA5 Antibody [NBP1-83352] - Analysis in human cell line U-87 MG.
Immunocytochemistry/Immunofluorescence: GOLGA5 Antibody [NBP1-83352] - Staining of human cell line U-2 OS shows localization to the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: GOLGA5 Antibody [NBP1-83352] - Staining of human liver.
(4 Publications)
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
GOSR1 Antibody
H00009527-B01P
Western Blot: GOSR1 Antibody [H00009527-B01P] - Analysis of GOSR1 expression in human liver.
Immunocytochemistry/Immunofluorescence: GOSR1 Antibody [H00009527-B01P] - Analysis of purified antibody to GOSR1 on HeLa cell. (antibody concentration 10 ug/ml)
Western Blot: GOSR1 Antibody [H00009527-B01P] - Analysis of GOSR1 expression in A-431.
(1 Review)
Species: Hu
Applications: WB, ELISA, ICC/IF
Syntaxin 5
Antibody [Unconjugate
AF5687
Syntaxin 5 was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Goat Anti-Human/Rat Syntaxin 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5687) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Syntaxin 5 was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Goat Anti-Human/Rat Syntaxin 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5687) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Syntaxin 5 was detected in immersion fixed frozen sections of rat brain using Goat Anti-Human/Rat Syntaxin 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5687) at 3 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Species: Hu, Rt
Applications: WB, Simple Western, IHC, ICC
beta COP Antibody
NBP2-15578
Western Blot: beta COP Antibody [NBP2-15578] - Sample (30 ug of whole cell lysate) A: Raji7. 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: beta COP Antibody [NBP2-15578] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:100 dilution.
Immunohistochemistry-Paraffin: beta COP Antibody [NBP2-15578] - COPB protein at cytoplasm in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. COPB antibody [N1N2], N-term diluted at 1:500.
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
GM130/GOLGA2
Antibody
H00002801-B02P
GM130/GOLGA2 Antibody
Species: Hu
Applications: WB, ICC/IF

Publications for GM130/GOLGA2 Antibody (H00002801-B02P) (0)

There are no publications for GM130/GOLGA2 Antibody (H00002801-B02P).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for GM130/GOLGA2 Antibody (H00002801-B02P) (0)

There are no reviews for GM130/GOLGA2 Antibody (H00002801-B02P). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GM130/GOLGA2 Antibody (H00002801-B02P) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

GM130/GOLGA2 Knockout HeLa Cell Lysate

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for GM130/GOLGA2 Antibody (H00002801-B02P)

Discover related pathways, diseases and genes to GM130/GOLGA2 Antibody (H00002801-B02P). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for GM130/GOLGA2 Antibody (H00002801-B02P)

Discover more about diseases related to GM130/GOLGA2 Antibody (H00002801-B02P).
 

Pathways for GM130/GOLGA2 Antibody (H00002801-B02P)

View related products by pathway.

PTMs for GM130/GOLGA2 Antibody (H00002801-B02P)

Learn more about PTMs related to GM130/GOLGA2 Antibody (H00002801-B02P).
 

Research Areas for GM130/GOLGA2 Antibody (H00002801-B02P)

Find related products by research area.

Blogs on GM130/GOLGA2

There are no specific blogs for GM130/GOLGA2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  PDF Datasheet
  Request CofA
  Request SDS

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our GM130/GOLGA2 Antibody and receive a gift card or discount.


Bioinformatics

Gene Symbol GOLGA2
Uniprot