GM130/GOLGA2 Antibody (2059C) [Unconjugated]

Western blot shows lysates of MCF-7 human breast cancer cell line, ZR-75 human breast cancer cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human GM130/GOLGA2 Monoclonal Antibody (Catalog # MAB81991) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for GM130/ GOLGA2 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, and ZR‑75 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for GM130/GOLGA2 at approximately 141 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human GM130/GOLGA2 Monoclonal Antibody (Catalog # MAB81991) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clone: 2059C
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

GM130/GOLGA2 Antibody (2059C) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: E. coli-derived recombinant human GM130/GOLGA2
  Met258-Gln606
  Accession # Q08379
• Specificity: Detects human GM130/GOLGA2 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: Sodium Azide
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GM130/GOLGA2 Antibody (2059C) [Unconjugated]

• 130 kDa cis-Golgi matrix protein
• GM130 autoantigen
• GM130
• GM130Golgi matrix protein GM130
• GOLGA2
• golgi autoantigen, golgin subfamily a, 2
• golgin A2
• Golgin subfamily A member 2
• Golgin-95
• MGC20672
• SY11 Protein

Background

GM130, also known as Golgin subfamily A member 2 and GOLGA2, is a 130 kDa member of the Golgin protein family.  GM130/GOLGA2 is a component of the Golgi membrane involved in maintaining cis-Golgi structure.  GM130/GOLGA2 protein is thought to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been predicted. Human GM130/GOLGA2 protein is 1002 amino acids (aa) in length, and over aa 528‑606, shares 90% sequence identity with its mouse and rat orthologs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GM130/GOLGA2 Antibody (MAB81991)(1)

Publication using MAB81991

• Nelson, TJ;Xu, Y; Sting and p53 DNA repair pathways are compromised in Alzheimer's disease Scientific reports 2023-05-23 [PMID: 37221295] (Western Blot, Human)

Bioinformatics

• Uniprot:
  • Q08379()