GM-CSFR alpha Antibody (JE61-37)

Western Blot: GM-CSFR alpha Antibody (JE61-37) [NBP3-32120] - Western blot analysis of GM-CSFR alpha on different lysates with Rabbit anti-GM-CSFR alpha antibody (NBP3-32120) at 1/500 dilution. Lane 1: JAR cell lysate (10 ug/Lane) Lane 2: Hela cell lysate (10 ug/Lane) Lane 3: 293T cell lysate (10 ug/Lane) Lane 4: Human placenta tissue lysate (20 ug/Lane) Predicted band size: 46 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32120) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: GM-CSFR alpha Antibody (JE61-37) [NBP3-32120] - Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue with Rabbit anti-GM-CSFR alpha antibody (NBP3-32120) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32120) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: GM-CSFR alpha Antibody (JE61-37) [NBP3-32120] - Immunocytochemistry analysis of SiHa cells labeling GM-CSFR alpha with Rabbit anti-GM-CSFR alpha antibody (NBP3-32120) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-GM-CSFR alpha antibody (NBP3-32120) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: JE61-37
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

GM-CSFR alpha Antibody (JE61-37) Summary

• Description: Novus Biologicals Rabbit GM-CSFR alpha Antibody (JE61-37) (NBP3-32120) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within Human GM-CSFR alpha aa 23-122 / 400. (Uniprot: P15509)
• Localization: Cell membrane; Secreted.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CSF2RA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000
  • Western Blot 1:500
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for GM-CSFR alpha Antibody (JE61-37)

• CD116 antigen
• CD116
• CDw116
• colony stimulating factor 2 receptor alpha subunit
• colony stimulating factor 2 receptor, alpha, low-affinity(granulocyte-macrophage)
• CSF2R
• CSF2RA
• CSF2RAX
• CSF2RAY
• CSF2RX
• CSF2RY
• GM-CSF R alpha
• GM-CSF receptor alpha subunit
• GMCSFR alpha
• GMCSFR
• GM-CSFRa
• GM-CSF-R-alpha
• GMCSFR-alpha
• GMR
• GMR-alpha
• granulocyte-macrophage colony-stimulating factor receptor alpha chain
• granulocyte-macrophage colony-stimulating factor receptor subunit alpha
• MGC3848
• MGC4838

Background

GM-CSF Receptor alpha is encoded by this gene is the alpha subunit of the heterodimeric receptor for colony stimulating factor 2, a cytokine which controls the production, differentiation, and function of granulocytes and macrophages. The encoded protein is a member of the cytokine family of receptors. This gene is found in the pseudoautosomal region (PAR) of the X and Y chromosomes. Multiple transcript variants encoding different isoforms have been found for this gene, with some of the isoforms being membrane-bound and others being soluble. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CSF2RA