| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | FAB706A | |||
| Carrier Free | MAB706-SP | |||
| Carrier Free | FAB706V-100UG | |||
| Carrier Free | FAB706U-100UG | |||
| Carrier Free | FAB706T-100UG | |||
| Carrier Free | FAB706S-100UG | |||
| Carrier Free | FAB706R-100UG | |||
| Carrier Free | FAB706N-100UG | |||
| Carrier Free | FAB706G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF706-SP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-43361B | |||
| HRP | NBP1-43361H | |||
![Western Blot: GM-CSFR alpha Antibody (JE61-37) [NBP3-32120] - Western blot analysis of GM-CSFR alpha on different lysates with Rabbit anti-GM-CSFR alpha antibody (NBP3-32120) at 1/500 dilution. Lane 1: JAR cell lysate (10 ug/Lane) Lane 2: Hela cell lysate (10 ug/Lane) Lane 3: 293T cell lysate (10 ug/Lane) Lane 4: Human placenta tissue lysate (20 ug/Lane) Predicted band size: 46 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32120) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32120_rabbit-gm-csfr-alpha-mab-je61-37-278202416331275.jpg "Western Blot: GM-CSFR alpha Antibody (JE61-37) [NBP3-32120] - Western blot analysis of GM-CSFR alpha on different lysates with Rabbit anti-GM-CSFR alpha antibody (NBP3-32120) at 1/500 dilution. Lane 1: JAR cell lysate (10 ug/Lane) Lane 2: Hela cell lysate (10 ug/Lane) Lane 3: 293T cell lysate (10 ug/Lane) Lane 4: Human placenta tissue lysate (20 ug/Lane) Predicted band size: 46 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32120) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: GM-CSFR alpha Antibody (JE61-37) [NBP3-32120] - Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue with Rabbit anti-GM-CSFR alpha antibody (NBP3-32120) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32120) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32120_rabbit-gm-csfr-alpha-mab-je61-37-298202414161813.jpg)
![Immunocytochemistry/ Immunofluorescence: GM-CSFR alpha Antibody (JE61-37) [NBP3-32120] - Immunocytochemistry analysis of SiHa cells labeling GM-CSFR alpha with Rabbit anti-GM-CSFR alpha antibody (NBP3-32120) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-GM-CSFR alpha antibody (NBP3-32120) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32120_rabbit-gm-csfr-alpha-mab-je61-37-29202410484890.jpg)
![Western Blot: GM-CSFR alpha Antibody (2G5) [H00001438-M03] - Analysis of CSF2RA expression in transfected 293T cell line by CSF2RA monoclonal antibody (M03), clone 2G5. Lane 1: CSF2RA transfected lysatE (46.2 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/GM-CSFR-alpha-Antibody-2G5-Western-Blot-H00001438-M03-img0003.jpg "Western Blot: GM-CSFR alpha Antibody (2G5) [H00001438-M03] - Analysis of CSF2RA expression in transfected 293T cell line by CSF2RA monoclonal antibody (M03), clone 2G5. Lane 1: CSF2RA transfected lysatE (46.2 KDa). Lane 2: Non-transfected lysate.")
![Proximity Ligation Assay: GM-CSFR alpha Antibody (2G5) [H00001438-M03] - PProximity Ligation Analysis (PLA) of protein-protein interactions between KIT and CSF2RA. HeLa cells were stained with anti-KIT rabbit purified polyclonal 1:1200 and anti-CSF2RA mouse monoclonal antibody 1:50. Signals were detected by Duolink (R) 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.represents the detection of protein-protein interaction complex.](http://images.novusbio.com/fullsize/GM-CSFR-alpha-Antibody-2G5-Proximity-Ligation-Assay-H00001438-M03-img0001.jpg)
![ELISA: GM-CSFR alpha Antibody (2G5) [H00001438-M03] - Detection limit for recombinant GST tagged CSF2RA is 3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/GM-CSFR-alpha-Antibody-2G5-ELISA-H00001438-M03-img0004.jpg)
![Flow Cytometry: GM-CSF R alpha Antibody (4H1) [NBP1-43361] - Analysis using the PE conjugate of NBP1-43361. Analysis of human peripheral blood leukocytes using GM-CSF R alpha PE conjugate. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8 cells/test.](http://images.novusbio.com/fullsize/GM-CSF-R-alpha-Antibody-4H1-Flow-Cytometry-NBP1-43361-img0004.jpg "Flow Cytometry: GM-CSF R alpha Antibody (4H1) [NBP1-43361] - Analysis using the PE conjugate of NBP1-43361. Analysis of human peripheral blood leukocytes using GM-CSF R alpha PE conjugate. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8 cells/test.")
![Flow Cytometry: GM-CSF R alpha Antibody (4H1) [NBP1-43361] - Surface staining of normal human peripheral blood cells with Anti-Human CD116 FITC (left) and PE (right). Appropriate isotype controls were used (open histogram). Cells in the monocyte population were used for analysis.](http://images.novusbio.com/fullsize/GM-CSF-R-alpha-Antibody-4H1-Flow-Cytometry-NBP1-43361-img0002.jpg)
![Flow Cytometry: GM-CSF R alpha Antibody (4H1) [NBP1-43361] - Human peripheral blood monocytes stained with 4H1 FITC.](http://images.novusbio.com/fullsize/GM-CSF-R-alpha-Antibody-4H1-Flow-Cytometry-NBP1-43361-img0003.jpg)
![Western Blot: GM-CSFR alpha Antibody [H00001438-B01P] - Analysis of CSF2RA expression in transfected 293T cell line by CSF2RA polyclonal antibody. Lane 1: CSF2RA transfected lysate(44 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/GM-CSFR-alpha-Antibody-Western-Blot-H00001438-B01P-img0004.jpg "Western Blot: GM-CSFR alpha Antibody [H00001438-B01P] - Analysis of CSF2RA expression in transfected 293T cell line by CSF2RA polyclonal antibody. Lane 1: CSF2RA transfected lysate(44 KDa). Lane 2: Non-transfected lysate.")
![Flow Cytometry: GM-CSFR alpha Antibody [H00001438-B01P] - Analysis of negative control 293 cells (Black) and CSF2RA expressing 293 cells (Green) using CSF2RA purified mouse polyclonal antibody.](http://images.novusbio.com/fullsize/GM-CSFR-alpha-Antibody-Flow-Cytometry-H00001438-B01P-img0003.jpg)
![Western Blot: GM-CSF Receptor alpha Overexpression Lysate (Adult Normal) [NBP2-11303] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GM-CSF Receptor alpha.](http://images.novusbio.com/fullsize/GM-CSF-Receptor-alpha-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11303-img0001.jpg "Western Blot: GM-CSF Receptor alpha Overexpression Lysate (Adult Normal) [NBP2-11303] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GM-CSF Receptor alpha.")
![Western Blot: GM-CSF Receptor alpha Overexpression Lysate (Adult Normal) [NBP2-11479] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GM-CSF Receptor alpha.](http://images.novusbio.com/fullsize/GM-CSF-Receptor-alpha-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11479-img0001.jpg "Western Blot: GM-CSF Receptor alpha Overexpression Lysate (Adult Normal) [NBP2-11479] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GM-CSF Receptor alpha.")
![Western Blot: GM-CSF Receptor alpha Overexpression Lysate (Adult Normal) [NBP2-04432] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GM-CSF Receptor alpha.](http://images.novusbio.com/fullsize/GM-CSF-Receptor-alpha-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04432-img0001.jpg "Western Blot: GM-CSF Receptor alpha Overexpression Lysate (Adult Normal) [NBP2-04432] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GM-CSF Receptor alpha.")