Glyoxalase I Antibody (CPTC-GLO1-1)

Western Blot: Glyoxalase I Antibody (CPTC-GLO1-1) [NBP3-07311] - Western Blot Analysis of HeLa cell lysate using Glyoxalase I Mouse Monoclonal Antibody (CPTC-GLO1-1).

Immunohistochemistry-Paraffin: Glyoxalase I Antibody (CPTC-GLO1-1) [NBP3-07311] - Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Glyoxalase I Mouse Monoclonal Antibody (CPTC-GLO1-1).

Immunohistochemistry-Paraffin: Glyoxalase I Antibody (CPTC-GLO1-1) [NBP3-07311] - Formalin-fixed, paraffin-embedded human thyroid carcinoma stained with Glyoxalase I Mouse Monoclonal Antibody (CPTC-GLO1-1).

Protein Array: Glyoxalase I Antibody (CPTC-GLO1-1) [NBP3-07311] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Glyoxalase I Monoclonal Antibody (CPTC-GLO1-1).

• Reactivity: Hu
• Applications: WB, IHC, MA
• Clone: CPTC-GLO1-1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Glyoxalase I Antibody (CPTC-GLO1-1) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08555)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant full-length human Glyoxalase I protein (Uniprot: Q04760)
• Localization: Cytoplasm
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GLO1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
  • Western Blot 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Glyoxalase I Antibody (CPTC-GLO1-1)

• Aldoketomutase
• EC 4.4.1.5
• GLO1
• GLOD1
• Glx I
• GLYI
• glyoxalase domain containing 1
• Glyoxalase I
• glyoxalase Ialdoketomutase
• Ketone-aldehyde mutase
• lactoyl glutathione lyase
• lactoylglutathione lyase
• Methylglyoxalase
• S-D-lactoylglutathione methylglyoxal lyase

Background

Lactoylglutathione lyase, also known as GLO1, is a member of the glyoxalase I family that is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. It is believed that inactivation of GLO1 in endothelial cells contributes to vascular complications in diabetes. GLO1 has also been shown to extend lifespan in C. elegans, and has been shown to be upregulated in malignant cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GLO1
• Uniprot:
  • Q04760(Human)