Glycophorin A Antibody (SPM599) - IHC-Prediluted

Immunohistochemistry-Paraffin: Glycophorin A Antibody (SPM599) - IHC-Prediluted [NBP2-48395] - Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Glycophorin A Monoclonal Antibody (SPM599)

Immunohistochemistry-Paraffin: Glycophorin A Antibody (SPM599) - IHC-Prediluted [NBP2-48395] - Formalin-fixed, paraffin-embedded human Placenta stained with Glycophorin A Monoclonal Antibody (SPM599)

Western Blot Analysis of K562 cell lysate using Glycophorin A Antibody (SPM599) - IHC-Prediluted.

• Reactivity: Hu
• Applications: IHC
• Clone: SPM599
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: IHC-Prediluted

Glycophorin A Antibody (SPM599) - IHC-Prediluted Summary

• Description: The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
• Immunogen: Recombinant human Glycophorin A protein (Uniprot: P02724)
• Localization: Cell surface
• Marker: Erythrocyte Marker
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GYPA
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for Glycophorin A Antibody (SPM599) - IHC-Prediluted

• CD235a antigen
• CD235a
• glycophorin A (includes MN blood group)
• glycophorin A (MNS blood group)
• Glycophorin A
• glycophorin Erik
• glycophorin MiI
• glycophorin MiIII
• glycophorin MiV
• glycophorin MiX
• glycophorin SAT
• glycophorin Sta type C
• glycophorin-A
• GPA
• GPErik
• GpMiIII
• GPSAT
• GYPA
• HGpMiIII
• HGpMiV
• HGpMiX
• HGpMiXI
• HGpSta(C)
• Mi.V glycoprotein (24 AA)
• MN sialoglycoprotein
• MNS
• PAS-2
• recombinant glycophorin A-B Miltenberger-DR

Background

Glycophorins A (GPA) and B (GPB) are single pass membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GYPA