Glutathione Peroxidase 4/GPX4 Antibody

Western Blot: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-76933] - Western blot analysis of GPX4 on rat testis tissue lysate using anti- GPX4 antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-76933] - ICC staining GPX4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-76933] - Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti- GPX4 antibody. Counter stained with hematoxylin.

Flow Cytometry: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-76933] - Flow cytometric analysis of HepG2 cells with GPX4 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-76933] - ICC staining GPX4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-76933] - ICC staining GPX4 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Glutathione Peroxidase 4/GPX4 Antibody Summary

• Description: Novus Biologicals Rabbit Glutathione Peroxidase 4/GPX4 Antibody (NBP2-76933) is a polyclonal antibody validated for use in IHC, WB, Flow and ICC/IF. Anti-Glutathione Peroxidase 4/GPX4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein within Human Glutathione Peroxidase 4/GPX4 aa 1-197 / 197. (SwissProt: P36969 Human; SwissProt: O70325 Mouse; SwissProt: P36970 Rat)
• Localization: Cytoplasm. Mitochondrion.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GPX4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50 -1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Glutathione Peroxidase 4/GPX4 Antibody

• EC 1.11.1
• EC 1.11.1.12
• glutathione peroxidase 4 (phospholipid hydroperoxidase)
• Glutathione Peroxidase 4
• GPX4
• GPx-4
• GSHPx-4
• MCSP
• PHGPx
• PHGPxsnGPx
• phospholipid hydroperoxidase
• phospholipid hydroperoxide glutathione peroxidase, mitochondrial
• snGPx
• snPHGPx
• sperm nucleus glutathione peroxidase

Background

Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, experimental evidence for alternative splicing exists; alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glutathione Peroxidase 4/GPX4 Antibody (NBP2-76933)(1)

Publication using NBP2-76933

• Su H, Cantrell A, Chen J et al. SIRT3 Deficiency Enhances Ferroptosis and Promotes Cardiac Fibrosis via p53 Acetylation Cells 2023-05-19 [PMID: 37408261] (Western Blot, Rat)

Bioinformatics

• Gene Symbol: GPX4