Glutathione Peroxidase 2/GPX2 Antibody (HL125) - Azide and BSA Free

Western Blot: Glutathione Peroxidase 2/GPX2 Antibody (HL125) [NBP3-13662] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Glutathione Peroxidase 2/GPX2 antibody [HL125] (NBP3-13662) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Glutathione Peroxidase 2/GPX2 Antibody (HL125) [NBP3-13662] - Glutathione Peroxidase 2/GPX2 antibody [HL125] detects Glutathione Peroxidase 2/GPX2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Glutathione Peroxidase 2/GPX2 stained by Glutathione Peroxidase 2/GPX2 antibody [HL125] (NBP3-13662) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: Glutathione Peroxidase 2/GPX2 Antibody (HL125) [NBP3-13662] - Glutathione Peroxidase 2/GPX2 antibody [HL125] detects Glutathione Peroxidase 2/GPX2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded dog kidney.Glutathione Peroxidase 2/GPX2 stained by Glutathione Peroxidase 2/GPX2 antibody [HL125] (NBP3-13662) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: Glutathione Peroxidase 2/GPX2 Antibody (HL125) [NBP3-13662] - Glutathione Peroxidase 2/GPX2 antibody [HL125] detects Glutathione Peroxidase 2/GPX2 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.Glutathione Peroxidase 2/GPX2 stained by Glutathione Peroxidase 2/GPX2 antibody [HL125] (NBP3-13662) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: Glutathione Peroxidase 2/GPX2 Antibody (HL125) [NBP3-13662] - Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with GPX2 antibody [HL125] diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Whole zebrafish extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with GPX2 antibody [HL125] (NBP3-13662) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with GPX2 antibody [HL125] (NBP3-13662) diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: HL125
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Glutathione Peroxidase 2/GPX2 Antibody (HL125) - Azide and BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Glutathione Peroxidase 2/GPX2. The exact sequence is proprietary.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GPX2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Glutathione Peroxidase 2/GPX2 Antibody (HL125) - Azide and BSA Free

• EC 1.11.1
• EC 1.11.1.9
• gastrointestinal glutathione peroxidase 2
• Gastrointestinal glutathione peroxidase
• GIGPX
• GI-GPx
• glutathione peroxidase 2 (gastrointestinal)
• Glutathione Peroxidase 2
• Glutathione peroxidase-gastrointestinal
• Glutathione peroxidase-related protein 2
• GPRP
• GPRP-2
• GPX2
• GPx-2
• GPx-GI
• GSHPx-2
• GSHPX-GI

Background

Glutathione Peroxidase 2 is a member of the glutathione peroxidase family and encodes a selenium-dependent glutathione peroxidase that is one of two isoenzymes responsible for the majority of the glutathione-dependent hydrogen peroxide-reducing activity in the epithelium of the gastrointestinal tract. Studies in knockout mice indicate that mRNA expression levels respond to luminal microflora, suggesting a role of the ileal glutathione peroxidases in preventing inflammation in the GI tract.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GPX2