Glutamine Synthetase Antibody

Product Discontinued

Glutamine Synthetase Antibody Summary

• Description: This antibody was prepared from monospecific antiserum by a delipidation and defibrination.
• Immunogen: Glutamine Synthetase [native from brevibacterium species]. Uniprot ID Q79VE3
• Localization: Cytoplasmic
• Specificity: Glutamine Synthetase
• Isotype: Serum
• Clonality: Polyclonal
• Host: Goat
• Gene: GLUL
• Purity: Delipidation and Defibrination

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:20000
  • Immunoprecipitation 1:100
  • Western Blot 1:500-1:5000

Reactivity Notes

Cross-reacts with E. coli. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Delipidation and Defibrination

Alternate Names for Glutamine Synthetase Antibody

• cell proliferation-inducing protein 59
• EC 4.1.1.15
• EC 6.3.1.2
• GLNSPIG59
• Glutamate decarboxylase
• glutamate-ammonia ligase (glutamine synthase)
• glutamate-ammonia ligase
• Glutamate--ammonia ligase
• glutamine synthase
• glutamine synthetase
• GS
• PIG43
• proliferation-inducing protein 43

Background

Glutamine Synthetase catalyzes the conversion of ammonia and glutamate to glutamine. This reaction consumes a molecule of ATP:Glutamate + NH4+ + ATP , and produces, Glutamine + ADP + Pi. It is found in astrocytes as an octamer of identical 45kDa subunits. The function of Glutamine Synthetase is the detoxification of brain ammonia. It also has an important role in controlling metabolic regulations of neurotransmitter glutamate. Because of the multiple functions and importance of Glutamine Synthetase in cellular metabolism, both catalytic activities and synthesis are highly regulated. The activity of Glutamine Synthetase is controlled by adenylylation. Its activity is decreased in the cerebral cortex of brains affected by Alzheimer's disease, particularly in the vicinity of senile plaques. It is also decreased under conditions of glucose deprivation. On the other hands, the level of expression of Glutamine Synthetase is increased during ischemia in vivo or hypoxia in culture.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GLUL
• Entrez:
  • 2752(Human)