Glutamate Receptor 3 Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

Glutamate Receptor 3 Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human GRIA3.
  
  

  Source: E. coli

  Amino Acid Sequence: NNWQVTARSVGNIKDVQEFRRIIEEMDRRQEKRYLIDCEVERINTILEQVVILGKHSRGYHYM

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: GRIA3
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-37972.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for Glutamate Receptor 3 Recombinant Protein Antigen

• AMPA 3
• AMPA3
• AMPA-selective glutamate receptor 3
• GluA3
• GluR3
• GluR-3
• GLURC
• GLUR-C
• GLUR-K3
• glutamate receptor C
• glutamate receptor subunit 3
• glutamate receptor, ionotrophic, AMPA 3
• GRIA3

Background

The GRIA3 gene encodes for glutamate receptor 3 with an isoform flop and flip of 894 amino acids, 101 kDA each. Glutamate receptors function as a ligand-gated ion channel in the central nervous system and are the predominant excitatory neurotransmitter receptors in the brain. GRIA3 is involved in glutamic acid signaling, calcium channels, CREB pathways, pathogenesis of ALS, and glutamatergic synapses. It is known to interact with GRIA2, DLG4, NSF, SQSTM1, and SDCBP. GRIA3 has been researched with various diseases such as bipolar disorder, encephalitis, mood disorder, rett syndrome, lateral sclerosis, auditory neuropathy, epilepsy, smith-fineman-myers syndrome, and nonsyndromic deafness, and canavan disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: GRIA3