Glutamate Dehydrogenase Antibody

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Western Blot: Glutamate Dehydrogenase Antibody [NB600-853] - Western blot analysis is shown using Glutamate Dehydrogenase antibody to detect the enzyme from bovine liver preparations. Comparison to a molecular weight ...read more
N/A: Glutamate Dehydrogenase Antibody [NB600-853]

Product Details

Summary
Reactivity Hu, BvSpecies Glossary
Applications WB, ELISA, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Glutamate Dehydrogenase Antibody Summary

Description
This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Glutamate Dehydrogenase [Bovine Liver]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Immunogen
This Glutamate Dehydrogenase Antibody was prepared from whole rabbit serum produced by repeated immunizations with a full length Glutamate Dehydrogenase protein isolated from Bovine Liver. (Uniprot: P00366)
Localization
Mitochondrial matrix
Marker
Mitochondria Marker
Isotype
Serum
Clonality
Polyclonal
Host
Rabbit
Gene
GLUD1
Purity
Delipidation and Defibrination
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:4000-1:16000
  • Immunohistochemistry
  • Immunoprecipitation 1:100
  • Western Blot 1:1000-1:3000
Application Notes
This antibody has been tested by western blot. Specific conditions for reactivity should be optimized by the end user. Bovine glutamate dehydrogenase exists as a homohexamer located within the mitochondrial matrix. Expect a band approximately 56 kDa in size corresponding to glutamate dehydrogenase monomer subunit by western blotting in the appropriate cell or tissue extract. Anti-Glutamate Dehydrogenase Antibody is suitable for use in ELISA.

Use in Immunohistochemistry reported in scientific literature (PMID 27623078).
Reviewed Applications
Read 1 Review rated 4
using
NB600-853 in the following applications:

Publications
Read Publications using
NB600-853 in the following applications:

Reactivity Notes

BLAST analysis was used to determine that cross reactivity is suggested for both mitochondrial and brain isoforms (GDH1 and GDH2), from both bovine and human sources. Additionally similar reactivity is suggested for most primate species including green monkey, white gibbon, chimpanzee orangutan, and gorilla. A high degree of sequence homology is also noted for Glutamate Dehydrogenase from chicken, mouse, rat, dog, and other mammals as well as Xenopus tropicalis, zebrafish, rainbow trout and Atlantic salmon.
Human reactivity reported in scientific literature (PMID: 27623078).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Delipidation and Defibrination

Alternate Names for Glutamate Dehydrogenase Antibody

  • EC 1.4.1
  • EC 1.4.1.3
  • GDH 1
  • GDH
  • GDH1
  • GLUD
  • glutamate dehydrogenase (NAD(P)+)
  • Glutamate Dehydrogenase 1
  • glutamate dehydrogenase 1, mitochondrial
  • MGC132003

Background

One enzyme central to the metabolism of glutamate is glutamate dehydrogenase (GDH1; EC 1.4.1.3), that catalyzes the reversible deamination of L-glutamate to 2-oxoglutarate using NAD+ or NADP+. Mammalian GDH is composed of six identical subunits, and the regulation of GDH is very complex. It has been a major goal to identify the substrate and regulatory binding sites of GDH. It is only in recent years that the three-dimensional structure of GDH from microorganisms is available. Very recently, crystallization of bovine liver GDH was reported for the first time from the mammalian sources. However, remarkably little is known about the detailed structure of mammalian GDH, especially the brain enzymes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glutamate Dehydrogenase Antibody (NB600-853)(4)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 2 applications: IF/IHC, WB.


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IF/IHC
(1)
WB
(2)
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Human
(2)
Rat
(1)
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Review for Glutamate Dehydrogenase Antibody (NB600-853) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Pig.

Reviews using NB600-853:
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WB
(1)
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Pig
(1)
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Images Ratings Applications Species Date Details
Western Blot Glutamate Dehydrogenase NB600-853
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4
reviewed by:
Paula Chen
WB Pig 01/09/2019
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Summary

ApplicationWestern Blot
Sample TestedPorcine embryo
SpeciesPig
Lot16721

Comments

CommentsA 4-20% gradient gel was used to separate proteins from pools of 40 blastocysts before transfer to PVDF membranes. The membrane was incubated in 1:1000 anti-glutamate dehydrogenase primary overnight at 4 degrees Celsius. The next day, membranes were incubated in 1:10000 anti-rabbit IgG HRP secondary for 1 hour.

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Secondary Antibodies

 

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Paula Chen
01/09/2019
Application: WB
Species: Pig

Bioinformatics

Gene Symbol GLUD1
Entrez
Uniprot