Glut4 [p Ser488] Antibody (JE43-23)

Western Blot: Glut4 [p Ser488] Antibody (JE43-23) [NBP3-32766] - Western blot analysis of Glut4 on mouse heart tissue lysates (no heat) with Rabbit anti-Glut4 antibody (NBP3-32766) at 1/1,000 dilution. Lysates/proteins at 30 ug/Lane. Predicted band size: 55 kDa Observed band size: 50 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32766) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: JE43-23
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Glut4 [p Ser488] Antibody (JE43-23) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser488 of Glut4. (Uniprot: P14672)
• Modification: p Ser488
• Localization: Cell membrane, Endomembrane system, Cytoplasm, perinuclear region.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SLC2A4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Glut4 [p Ser488] Antibody (JE43-23)

• Glut4
• GLUT-4
• GLUT4Glucose transporter type 4, insulin-responsive
• insulin-responsive glucose transporter type 4
• SLC2A4
• solute carrier family 2 (facilitated glucose transporter), member 4
• solute carrier family 2, facilitated glucose transporter member 4

Background

The stimulation of glucose uptake by insulin in muscle and adipose tissue requires translocation of the GLUT4 glucose transporter from intracellular storage sites to the cell surface. Activation of phosphatidylinositol-3-OH kinase (PI3K) is required for this trafficking event, but it is not sufficient to produce GLUT4 translocation. Human insulin resistance involves a defect in GLUT4 traffic and targeting leading to accumulation in a dense membrane compartment from which insulin is unable to recruit GLUT4 to the cell surface.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC2A4