Glut2 Antibody (199017) [Phycoerythrin]

HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Glut2 PE-conjugated Monoclonal Antibody (Catalog # FAB1414P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 199017
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

Glut2 Antibody (199017) [Phycoerythrin] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human Glut2
  Met1-Val524
  Accession # P11168
• Specificity: Recognizes human Glut2 expression on human Glut2‑transfected NS0 cells, but not on control transfectants. Was shown to detect Glut2 on the surface of the human intestinal cell line Caco2 (2) in flow cytometry and on fixed cells in immunocytochemistry tests. Based on flow cytometric tests on transfected cells, this antibody has no cross-reactivity with human Glut1 or human Glut3.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC2A2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glut2 Antibody (199017) [Phycoerythrin]

• GLUT2 Glucose transporter type 2, liver
• Glut2
• GLUT-2
• SLC2A2
• solute carrier family 2 (facilitated glucose transporter), member 2
• solute carrier family 2, facilitated glucose transporter member 2

Background

Glut2 belongs to the facilitative glucose transporter protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains. Glut2 is expressed predominantly in liver, intestine, kidney, and pancreatic beta-cells. It is a low-affinity glucose transporter that has been suggested to function as a glucose sensor in pancreatic beta-cells and facilitate either glucose uptake or efflux from cells depending on the nutritional state (1).

1. Olson, A.L. and J.E. Pessin (1996) Annu. Rev. Nut. 16:235.
2. Mahraoui, L. et al. (1994) J. Biochem. 298:629.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glut2 Antibody (FAB1414P)(6)

Publications using FAB1414P

• Zhao, Y;Veysman, B;Antolijao, K;Zhao, Y;Papagni, Y;Wang, H;Ross, R;Tibbot, T;Povrzenic, D;Fox, R; Increase in the Expression of Glucose Transporter 2 (GLUT2) on the Peripheral Blood Insulin-Producing Cells (PB-IPC) in Type 1 Diabetic Patients after Receiving Stem Cell Educator Therapy International journal of molecular sciences 2024-07-30 [PMID: 39125908] (Flow Cytometry, Human)
• S Sharari, B Kabeer, I Mohammed, B Haris, I Pavlovski, I Hawari, AA Bhat, M Toufiq, S Tomei, R Mathew, N Syed, S Nisar, S Maacha, JC Grivel, D Chaussabel, J Ericsson, K Hussain Understanding the Role of GLUT2 in Dysglycemia Associated with Fanconi-Bickel Syndrome Biomedicines, 2022-08-29;10(9):. 2022-08-29 [PMID: 36140215] (Flow Cytometry, Human)
• H Yu, W Hu, X Song, Y Zhao Generation of Multipotent Stem Cells from Adult Human Peripheral Blood Following the Treatment with Platelet-Derived Mitochondria Cells, 2020-05-29;9(6):. 2020-05-29 [PMID: 32485922] (Flow Cytometry, Human)
• CP Hasilo, S Negi, I Allaeys, N Cloutier, AK Rutman, M Gasparrini, É Bonneil, P Thibault, É Boilard, S Paraskevas Presence of diabetes autoantigens in extracellular vesicles derived from human islets Sci Rep, 2017-07-10;7(1):5000. 2017-07-10 [PMID: 28694505] (Flow Cytometry, Human)
• Ana F. Brito, Ana M. Abrantes, Marina Ribeiro, Rui Oliveira, João Casalta-Lopes, Ana C. Gonçalves et al. Fluorine-18 Fluorodeoxyglucose Uptake in Hepatocellular Carcinoma: Correlation with Glucose Transporters and p53 Expression Journal of Clinical and Experimental Hepatology 9/1/2015 [PMID: 26628835]
• Michau A, Guillemain G, Grosfeld A, Vuillaumier-Barrot S, Grand T, Keck M, L'Hoste S, Chateau D, Serradas P, Teulon J, De Lonlay P, Scharfmann R, Brot-Laroche E, Leturque A, Le Gall M Mutations in SLC2A2 gene reveal hGLUT2 function in pancreatic beta cell development. J Biol Chem, 2013-08-28;288(43):31080-92. 2013-08-28 [PMID: 23986439] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: SLC2A2
• Uniprot:
  • P11168()