Glucuronosyltransferase 1A1/UGT1A1 Antibody

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Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Glucuronosyltransferase 1A1/UGT1A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6490) followed by ...read more
Glucuronosyltransferase 1A1/UGT1A1 was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human Glucuronosyltransferase 1A1/UGT1A1 Antigen Affinity-purified Polyclonal Antibody ...read more
Glucuronosyltransferase 1A1/UGT1A1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human Glucuronosyltransferase 1A1/UGT1A1 Antigen Affinity-purified Polyclonal Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

Glucuronosyltransferase 1A1/UGT1A1 Antibody Summary

Immunogen
E. coli-derived recombinant human Glucuronosyltransferase 1A1/UGT1A1
Leu60-Thr168
Accession # P22309
Specificity
Detects human Glucuronosyltransferase 1A1/UGT1A1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
UGT1A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunocytochemistry 5-15 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glucuronosyltransferase 1A1/UGT1A1 Antibody

  • bilirubin UDP-glucuronosyltransferase 1-1
  • bilirubin UDP-glucuronosyltransferase isozyme 1
  • Bilirubin-specific UDPGT isozyme 1
  • GNT1
  • GNT1EC 2.4.1.17
  • HUG-BR1
  • UDP glucuronosyltransferase 1 family, polypeptide A1
  • UDP glycosyltransferase 1 family, polypeptide A1
  • UDP-glucuronosyltransferase 1-1
  • UDP-glucuronosyltransferase 1-A
  • UDP-glucuronosyltransferase 1A1
  • UDPGT 1-1
  • UDPGT
  • UG-BR1
  • UGT1
  • UGT1*1
  • UGT1.1
  • UGT1-01
  • UGT1A
  • UGT-1A
  • UGT1A1
  • UGT1AUDPGT 1-1
  • UGT1BILIQTL1

Background

UGT1A1 (UDP-glucuronosyltransferase 1-A1; also UDPGT 1-1 and HUG-BR1) is a 52-57 kDa member of the UGT1A subfamily, UGT family of enzymes. UGT1A1 is expressed by the liver, and catalyzes the conjugation of glucuronic acid (GA) from UDPGA to lipophilic acceptors such as (anti-cancer) drugs and bilirubin. Addition of glucuronic acid increases target solubility and facilitates elimination. Mature human UGT1A1 is a 508 amino acid (aa) type I transmembrane ER glycoprotein. It contains a 465 aa luminal domain (aa 26-490) plus a 26 aa cytoplasmic region. The luminal domain is unusual in that aa 157-176 are embedded in the ER membrane. Amino acids 29-444 contain the enzyme active site. The cytoplasmic tail appears to mediate noncovalent homodimerization, and heterodimerization with UGT2B. The signal sequence (aa 1-25) is normally cleaved, but a Lys15Arg mutation blocks insertion into the ER membrane. There are multiple point mutations that impact enzyme activity. One potential splice form is reported that shows a six aa substitution for aa 289-533. Over aa 60-186, human UGT1A1 shares 64% aa identity with mouse UGT1A1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol UGT1A1
Entrez
Uniprot